Baculoviruses are enveloped nucleocapsids which mature both in the nucleus and at the cell surface. The acquisition of an envelope in the cell nucleus requires the synthesis or transport of large quantities of viral envelope substrates to the nucleoplasm. The extent of synthesis and nature of budding of the baculovirus nucleocapsid in the nucleus is a unique phenomenon compared to other virus envelope maturation strategies. Comparison of the structural proteins of the nuclear and cytoplasmic viral forms show major differences in number, size, and in glycosylation and phosphorylation modifications. Studies of the viral genes and their encoded proteins which are unique to either viral form should provide important insights on the molecular basis of viral maturation in the nucleus. The studies proposed are: (a) to map and sequence envelope and nucleocapsid genes that encode proteins that reflect characteristics specific to each form of the baculovirus Autographa californica and which show potential for use as markers; (b) to use these characteristics to study expression, regulation of expression, processing, trafficking and assembly in infected cells. Emphasis will be on the origin and assembly of viral envelopes and the viral maturation process in the nucleus.

Agency
National Institute of Health (NIH)
Institute
National Institute of General Medical Sciences (NIGMS)
Type
Research Project (R01)
Project #
5R01GM047552-02
Application #
3307079
Study Section
Experimental Virology Study Section (EVR)
Project Start
1992-05-01
Project End
1995-04-30
Budget Start
1993-05-01
Budget End
1994-04-30
Support Year
2
Fiscal Year
1993
Total Cost
Indirect Cost
Name
Texas Agrilife Research
Department
Type
Schools of Earth Sciences/Natur
DUNS #
110521739
City
College Station
State
TX
Country
United States
Zip Code
77843
Braunagel, S C; Guidry, P A; Rosas-Acosta, G et al. (2001) Identification of BV/ODV-C42, an Autographa californica nucleopolyhedrovirus orf101-encoded structural protein detected in infected-cell complexes with ODV-EC27 and p78/83. J Virol 75:12331-8
Rosas-Acosta, G; Braunagel, S C; Summers, M D (2001) Effects of deletion and overexpression of the Autographa californica nuclear polyhedrosis virus FP25K gene on synthesis of two occlusion-derived virus envelope proteins and their transport into virus-induced intranuclear membranes. J Virol 75:10829-42
Braunagel, S C; Burks, J K; Rosas-Acosta, G et al. (1999) Mutations within the Autographa californica nucleopolyhedrovirus FP25K gene decrease the accumulation of ODV-E66 and alter its intranuclear transport. J Virol 73:8559-70
Beniya, H; Braunagel, S C; Summers, M D (1998) Autographa californica nuclear polyhedrosis virus: subcellular localization and protein trafficking of BV/ODV-E26 to intranuclear membranes and viral envelopes. Virology 240:64-75
Belyavskyi, M; Braunagel, S C; Summers, M D (1998) The structural protein ODV-EC27 of Autographa californica nucleopolyhedrovirus is a multifunctional viral cyclin. Proc Natl Acad Sci U S A 95:11205-10
Braunagel, S C; Parr, R; Belyavskyi, M et al. (1998) Autographa californica nucleopolyhedrovirus infection results in Sf9 cell cycle arrest at G2/M phase. Virology 244:195-211
Hong, T; Summers, M D; Braunagel, S C (1997) N-terminal sequences from Autographa californica nuclear polyhedrosis virus envelope proteins ODV-E66 and ODV-E25 are sufficient to direct reporter proteins to the nuclear envelope, intranuclear microvesicles and the envelope of occlusion derived virus. Proc Natl Acad Sci U S A 94:4050-5
Braunagel, S C; Elton, D M; Ma, H et al. (1996) Identification and analysis of an Autographa californica nuclear polyhedrosis virus structural protein of the occlusion-derived virus envelope: ODV-E56. Virology 217:97-110
Harrison, R L; Jarvis, D L; Summers, M D (1996) The role of the AcMNPV 25K gene, ""FP25,"" in baculovirus polh and p10 expression. Virology 226:34-46
Braunagel, S C; He, H; Ramamurthy, P et al. (1996) Transcription, translation, and cellular localization of three Autographa californica nuclear polyhedrosis virus structural proteins: ODV-E18, ODV-E35, and ODV-EC27. Virology 222:100-14

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