The long term goal of this application is to understand the biological function of type XVI collagen. Collagens, which are major structural macromolecules of connective tissue, consist of at least 19 distinct types. Type XVI collagen is a recently discovered member of the collagen family. It possesses several structural features characteristic of the fibril associated collagen subgroup, whose members include type IS, XII, XIV, and XIX collagens. However, it is unique among collagens in that its long collagenous region is interrupted by multiple noncollagenous domains enriched in cysteines. Type XVI collagen mRNA is expressed at low levels in most tissues but at much higher levels in overy, testis and prosate tissue, suggesting that type XVI collagen plays critical roles in male and female reproductive organs. In this renewal application, experiemtns are proposed to continue investigating the function of type XVI collagen. The expression of type XVI collagenmRNa and protein in male and female reproductive organs will be examined in detail. Transgenic mice with the type XVI collagen gene inactivated or mutated will be prepared to study the in vivo function of type XVI collagen. Recombinant type XVI collagen chains will be prepared to study their binding interactions with other extracellular matrix proteins. The transcription regulation type XVI collagen gene will be studied by characterization of the promoter region of the gene.

Agency
National Institute of Health (NIH)
Institute
National Institute of General Medical Sciences (NIGMS)
Type
Research Project (R01)
Project #
5R01GM048532-06
Application #
2685012
Study Section
Special Emphasis Panel (ZRG3-BIO (01))
Project Start
1993-01-01
Project End
2001-03-31
Budget Start
1998-04-01
Budget End
1999-03-31
Support Year
6
Fiscal Year
1998
Total Cost
Indirect Cost
Name
Thomas Jefferson University
Department
Dermatology
Type
Schools of Medicine
DUNS #
061197161
City
Philadelphia
State
PA
Country
United States
Zip Code
19107
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