Specific Aims: l. To identify and characterize additional members of the Lrp regulon. 2. To determine whether there are lrp-like genes in organisms other than enteric bacteria that have a major global regulatory function. 3. To explore how Lrp activates transcription from the ilvIH promoter, and how leucine interferes with activation. 4. To investigate how leucine can overcome the effect of Lrp in some cases, potentiate the effect in other cases, and have no effect in yet other cases. 5. To determine the three dimensional structure of Lrp and of Lrp-DNA complexes. 6. To test the hypothesis that small changes in Lrp concentration underlie major changes in metabolism that occur in E. coli when cells make a transition from one medium to another.

Agency
National Institute of Health (NIH)
Institute
National Institute of General Medical Sciences (NIGMS)
Type
Research Project (R01)
Project #
5R01GM048861-06
Application #
2668480
Study Section
Microbial Physiology and Genetics Subcommittee 2 (MBC)
Project Start
1993-02-01
Project End
2001-02-28
Budget Start
1998-03-01
Budget End
1999-02-28
Support Year
6
Fiscal Year
1998
Total Cost
Indirect Cost
Name
Cornell University
Department
Biochemistry
Type
Schools of Arts and Sciences
DUNS #
City
Ithaca
State
NY
Country
United States
Zip Code
14850
Wang, Q; Wu, J; Friedberg, D et al. (1994) Regulation of the Escherichia coli lrp gene. J Bacteriol 176:1831-9