Abstract

The overall goal is to establish the pathway for vacuole partitioning from mother cells to daughter cells. She plans to identify the macromolecules involved, to study how these components interact, and to determine how they are regulated.
Specific aims are: (1) development of new techniques for the isolation of yeast mutants defective in vacuole segregation (vac mutants); (2) characterization of the vacuole segregation mutants; (3) analysis of the timing of vacuole segregation and examination of vacuolar changes that precede the formation of the segregation structure; (4) utilization of the vac mutants to clone the corresponding wild-type (VAC) genes; (5) sequence analysis and genetic disruption of genes required for vacuole segregation; and (6) characterization of VAC-encoded proteins.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Institute of General Medical Sciences (NIGMS)
Type
Research Project (R01)
Project #
5R01GM050403-02
Application #
2188226
Study Section
Cellular Biology and Physiology Subcommittee 1 (CBY)
Project Start
1994-01-01
Project End
1997-12-31
Budget Start
1995-01-01
Budget End
1995-12-31
Support Year
2
Fiscal Year
1995
Total Cost
Indirect Cost

  Institution

Name
University of Iowa
Department
Biochemistry
Type
Schools of Medicine
DUNS #
041294109
City
Iowa City
State
IA
Country
United States
Zip Code
52242

  Publications

Lang, Michael J; Strunk, Bethany S; Azad, Nadia et al. (2017) An intramolecular interaction within the lipid kinase Fab1 regulates cellular phosphatidylinositol 3,5-bisphosphate lipid levels. Mol Biol Cell 28:858-864
Jin, Natsuko; Jin, Yui; Weisman, Lois S (2017) Early protection to stress mediated by CDK-dependent PI3,5P2 signaling from the vacuole/lysosome. J Cell Biol 216:2075-2090
Hasegawa, Junya; Strunk, Bethany S; Weisman, Lois S (2017) PI5P and PI(3,5)P2: Minor, but Essential Phosphoinositides. Cell Struct Funct 42:49-60
Kim, Seong M; Roy, Saurabh G; Chen, Bin et al. (2016) Targeting cancer metabolism by simultaneously disrupting parallel nutrient access pathways. J Clin Invest 126:4088-4102
Jin, Natsuko; Lang, Michael J; Weisman, Lois S (2016) Phosphatidylinositol 3,5-bisphosphate: regulation of cellular events in space and time. Biochem Soc Trans 44:177-84
Jin, Yui; Strunk, Bethany S; Weisman, Lois S (2015) Close encounters of the lysosome-peroxisome kind. Cell 161:197-8
McCartney, Amber J; Zolov, Sergey N; Kauffman, Emily J et al. (2014) Activity-dependent PI(3,5)P2 synthesis controls AMPA receptor trafficking during synaptic depression. Proc Natl Acad Sci U S A 111:E4896-905
Li, Sheena Claire; Diakov, Theodore T; Xu, Tao et al. (2014) The signaling lipid PI(3,5)P? stabilizes V?-V(o) sector interactions and activates the V-ATPase. Mol Biol Cell 25:1251-62
McCartney, Amber J; Zhang, Yanling; Weisman, Lois S (2014) Phosphatidylinositol 3,5-bisphosphate: low abundance, high significance. Bioessays 36:52-64
Jin, Natsuko; Mao, Kai; Jin, Yui et al. (2014) Roles for PI(3,5)P2 in nutrient sensing through TORC1. Mol Biol Cell 25:1171-85

Showing the most recent 10 out of 37 publications