Agency
National Institute of Health (NIH)
Institute
National Institute of General Medical Sciences (NIGMS)
Type
Research Project (R01)
Project #
5R01GM051196-10
Application #
2189552
Study Section
Pharmacology A Study Section (PHRA)
Project Start
1985-04-01
Project End
1998-11-30
Budget Start
1995-12-01
Budget End
1996-11-30
Support Year
10
Fiscal Year
1996
Total Cost
Indirect Cost
Name
University of Pennsylvania
Department
Pharmacology
Type
Schools of Medicine
DUNS #
042250712
City
Philadelphia
State
PA
Country
United States
Zip Code
19104
Siehler, Sandra; Manning, David R (2002) Pathways of transduction engaged by sphingosine 1-phosphate through G protein-coupled receptors. Biochim Biophys Acta 1582:94-9
Chen, C A; Manning, D R (2001) Regulation of G proteins by covalent modification. Oncogene 20:1643-52
Windh, R T; Lee, M J; Hla, T et al. (1999) Differential coupling of the sphingosine 1-phosphate receptors Edg-1, Edg-3, and H218/Edg-5 to the G(i), G(q), and G(12) families of heterotrimeric G proteins. J Biol Chem 274:27351-8
Shahrestanifar, M; Fan, X; Manning, D R (1999) Lysophosphatidic acid activates NF-kappaB in fibroblasts. A requirement for multiple inputs. J Biol Chem 274:3828-33
Barr, A J; Brass, L F; Manning, D R (1997) Reconstitution of receptors and GTP-binding regulatory proteins (G proteins) in Sf9 cells. A direct evaluation of selectivity in receptor.G protein coupling. J Biol Chem 272:2223-9
Barr, A J; Manning, D R (1997) Agonist-independent activation of Gz by the 5-hydroxytryptamine1A receptor co-expressed in Spodoptera frugiperda cells. Distinguishing inverse agonists from neutral antagonists. J Biol Chem 272:32979-87
Cowen, D S; Molinoff, P B; Manning, D R (1997) 5-hydroxytryptamine1A receptor-mediated increases in receptor expression and activation of nuclear factor-kappaB in transfected Chinese hamster ovary cells. Mol Pharmacol 52:221-6
Cowen, D S; Sowers, R S; Manning, D R (1996) Activation of a mitogen-activated protein kinase (ERK2) by the 5-hydroxytryptamine1A receptor is sensitive not only to inhibitors of phosphatidylinositol 3-kinase, but to an inhibitor of phosphatidylcholine hydrolysis. J Biol Chem 271:22297-300
Butkerait, P; Zheng, Y; Hallak, H et al. (1995) Expression of the human 5-hydroxytryptamine1A receptor in Sf9 cells. Reconstitution of a coupled phenotype by co-expression of mammalian G protein subunits. J Biol Chem 270:18691-9