Molecular umbrellas represent a unique class of amphiphiles that has proven capable of transporting certain hydrophilic peptides across phospholipid bilayers without damaging the integrity of the membrane. Very recently, molecular umbrellas have also been found capable of (i) transporting a 16-mer oligonucleotide across cholesterol-rich phospholipid bilayers, (ii) increasing the water- solubility and hydrolytic stability of a hydrophobic drug, and inhibiting the binding of HIV and HSV to mammalian cells.
The specific aims of this research build upon these latest findings. In particular, this research is intended to provide a framework of understanding (i.e., to develop the basic science) that will allow for the rational exploitation of these findings. Specifically, the goals of this research are aimed at (i) clarifying how the overall length of a molecular umbrella, the spacing between facially amphiphilic units and an attached oligonucleotide, its shielding capacity, the degree of facial hydrophilicity, and the presence of facial guanidinium group affect its ability to transport an oligonucleotide across cholesterol-rich phospholipid bilayers, (ii) testing the feasibility of creating molecular umbrella conjugates of Amphotericin B that exhibit high critical micelle concentrations, high hydrolytic stability and high membrane selectivity, and (iii) defining the consequences of the size of persulfated molecular umbrellas on bilayer transport and on antiviral activity. The long-term objective of this program is to devise fundamentally new approaches to drug delivery based the use of molecular umbrellas. ? ?

Public Health Relevance

One of the major challenges in medicinal chemistry is to find ways of transporting drugs, effectively, into cells so that they can reach their intended target without damaging the integrity of the membrane. In particular, the outer """"""""skin"""""""" of cells has proven to be a significant barrier that has limited the access of many water-soluble drugs. This research is aimed at finding a general solution to this problem by designing molecules that can carry drugs across such barriers. This research has the potential for leading to new and more effective drugs that could be used to treat a wide range of diseases; e.g., cancer, HIV, Herpes virus and fungal infections. ? ? ?

Agency
National Institute of Health (NIH)
Institute
National Institute of General Medical Sciences (NIGMS)
Type
Research Project (R01)
Project #
5R01GM051814-14
Application #
7478000
Study Section
Synthetic and Biological Chemistry A Study Section (SBCA)
Program Officer
Chin, Jean
Project Start
1994-12-01
Project End
2010-07-31
Budget Start
2008-08-01
Budget End
2009-07-31
Support Year
14
Fiscal Year
2008
Total Cost
$284,602
Indirect Cost
Name
Lehigh University
Department
Chemistry
Type
Schools of Arts and Sciences
DUNS #
808264444
City
Bethlehem
State
PA
Country
United States
Zip Code
18015
Janout, Vaclav; Cline, Lauren L; Feuston, Bradley P et al. (2014) Molecular umbrella conjugate for the ocular delivery of siRNA. Bioconjug Chem 25:197-201
Cline, Lauren L; Janout, Vaclav; Fisher, Michael et al. (2011) A molecular umbrella approach to the intracellular delivery of small interfering RNA. Bioconjug Chem 22:2210-6
Petrov, Ravil R; Chen, Wen-Hua; Regen, Steven L (2009) Thermally gated liposomes: a closer look. Bioconjug Chem 20:1037-43
Mehiri, Mohamed; Chen, Wen-Hua; Janout, Vaclav et al. (2009) Molecular umbrella transport: exceptions to the classic size/lipophilicity rule. J Am Chem Soc 131:1338-9
Chen, Wen-Hua; Janout, Vaclav; Kondo, Masaharu et al. (2009) A fine line between molecular umbrella transport and ionophoric activity. Bioconjug Chem 20:1711-5
Janout, Vaclav; Regen, Steven L (2009) Bioconjugate-based molecular umbrellas. Bioconjug Chem 20:183-92
Kondo, Masaharu; Mehiri, Mohamed; Regen, Steven L (2008) Viewing membrane-bound molecular umbrellas by parallax analyses. J Am Chem Soc 130:13771-7
Mehiri, Mohamed; Jing, Bingwen; Ringhoff, Danielle et al. (2008) Cellular entry and nuclear targeting by a highly anionic molecular umbrella. Bioconjug Chem 19:1510-3
Liu, Guozheng; Hnatowich, Donald J (2008) A semiempirical model of tumor pretargeting. Bioconjug Chem 19:2095-104
Madan, Rebecca Pellett; Mesquita, Pedro M M; Cheshenko, Natalia et al. (2007) Molecular umbrellas: a novel class of candidate topical microbicides to prevent human immunodeficiency virus and herpes simplex virus infections. J Virol 81:7636-46

Showing the most recent 10 out of 21 publications