The dsDNA bacteriophages are the most numerous taxon of evolving organisms in the biosphere. Information currently available from genomic sequencing of these organisms has revealed important insights into their evolution, and has given good reason to believe that a modest amount of additional sequence determination of whole genomes will substantially augment our understanding of the genetic structures of phage populations and of their evolutionary mechanisms and history. We propose to determine the sequences of nine bacteriophage genomes, three of the lambdoid family, four from the mycobacteriophages, and two others. We will analyze and compare the sequences to elucidate their genetic organizations and to make evolutionary inferences about the phages. In addition to providing evolutionary information for the bacteriophages, this project is expected to contribute to basic research on bacteriophage biology by providing comparative data about gene organization and function as well as by making new bacteriophages available for detailed experimentation. In addition, sequence data from this project is expected to assist in using bacteriophages to analyze and counteract human diseases caused by their hosts, specifically including tuberculosis.

National Institute of Health (NIH)
National Institute of General Medical Sciences (NIGMS)
Research Project (R01)
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Microbial Physiology and Genetics Subcommittee 2 (MBC)
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University of Pittsburgh
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Pope, Welkin H; Bowman, Charles A; Russell, Daniel A et al. (2015) Whole genome comparison of a large collection of mycobacteriophages reveals a continuum of phage genetic diversity. Elife 4:e06416
Petrova, Zaritza O; Broussard, Gregory W; Hatfull, Graham F (2015) Mycobacteriophage-repressor-mediated immunity as a selectable genetic marker: Adephagia and BPs repressor selection. Microbiology 161:1539-51
Cresawn, Steven G; Pope, Welkin H; Jacobs-Sera, Deborah et al. (2015) Comparative genomics of Cluster O mycobacteriophages. PLoS One 10:e0118725
Casjens, Sherwood R; Jacobs-Sera, Deborah; Hatfull, Graham F et al. (2015) Genome Sequence of Salmonella enterica Phage Det7. Genome Announc 3:
Hendrix, Roger W; Ko, Ching-Chung; Jacobs-Sera, Deborah et al. (2015) Genome Sequence of Salmonella Phage ?. Genome Announc 3:
Hatfull, Graham F (2014) Molecular Genetics of Mycobacteriophages. Microbiol Spectr 2:
Pietilä, Maija K; Laurinmäki, Pasi; Russell, Daniel A et al. (2013) Structure of the archaeal head-tailed virus HSTV-1 completes the HK97 fold story. Proc Natl Acad Sci U S A 110:10604-9
Sen?ilo, Ana; Jacobs-Sera, Deborah; Russell, Daniel A et al. (2013) Snapshot of haloarchaeal tailed virus genomes. RNA Biol 10:803-16
Pope, Welkin H; Jacobs-Sera, Deborah; Best, Aaron A et al. (2013) Cluster J mycobacteriophages: intron splicing in capsid and tail genes. PLoS One 8:e69273
Hatfull, Graham F; Science Education Alliance Phage Hunters Advancing Genomics and Evolutionary Science Program; KwaZulu-Natal Research Institute for Tuberculosis and HIV Mycobacterial Genetics Course Students et al. (2012) Complete genome sequences of 138 mycobacteriophages. J Virol 86:2382-4

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