Fis is a small DNA binding protein that plays an important role in a number of cellular processes in E. coli and other enterics, including site-specific recombination systems (e.g. Hin and Gin) and transcription initiation (e.g. rRNA and some tRNA promoters). Its regulation pattern is interesting, with synthesis dramatically increasing from undetectable to 25,000-50,000 molecules/cell following upshift. The goals of this grant are (1) to understand the mechanism of regulation of fis transcription, focusing primarily on the role of initiating nucleotide concentrations (CTP), IHF, and Fis itself on the activity of the fis promoter; (2) to determine what other protein products are controlled by Fis; and (3) to understand the determinants of DNA recognition by Fis.

Agency
National Institute of Health (NIH)
Institute
National Institute of General Medical Sciences (NIGMS)
Type
Research Project (R01)
Project #
2R01GM052051-06A1
Application #
6193054
Study Section
Microbial Physiology and Genetics Subcommittee 2 (MBC)
Program Officer
Tompkins, Laurie
Project Start
1995-01-01
Project End
2004-06-30
Budget Start
2000-07-01
Budget End
2001-06-30
Support Year
6
Fiscal Year
2000
Total Cost
$242,415
Indirect Cost
Name
State University of New York at Albany
Department
Biology
Type
Schools of Arts and Sciences
DUNS #
City
Albany
State
NY
Country
United States
Zip Code
12222
Shao, Yongping; Feldman-Cohen, Leah S; Osuna, Robert (2008) Biochemical identification of base and phosphate contacts between Fis and a high-affinity DNA binding site. J Mol Biol 380:327-39
Shao, Yongping; Feldman-Cohen, Leah S; Osuna, Robert (2008) Functional characterization of the Escherichia coli Fis-DNA binding sequence. J Mol Biol 376:771-85
Du, Qiang; Park, Kyung Soo; Guo, Zhong et al. (2006) Regulation of human nitric oxide synthase 2 expression by Wnt beta-catenin signaling. Cancer Res 66:7024-31