The goal of the experiments described in this proposal is to characterize the cellular and molecular mechanisms underlying coordinated microtubule-actomyosin interaction. We will employ three model systems to understand how these two protein polymer systems direct the rapid establishment of transient cytoskeletal arrays: 1) Xenopus oocyte wound healing; 2) Cytokinesis in Xenopus embryos; 3) Nuclear anchoring and meiotic spindle assembly in Xenopus eggs. For the first two model systems, we will ask how localized signals that direct recruitment of specific players are generated, and how microtubules control those signals. For the last model system, we will ask whether a molecular motor-myosin-10- works as multifunctional crosslinker. To answer these questions, we will use a combination of molecular biology, high resolution live cell imaging, and biochemistry. The successful completion of the proposed experiments will not only answer basic questions in biology, it will also provide critical insights into processes such as wound repair, cancer, birth defects and human infertility. ? ?
| Sandquist, Joshua C; Larson, Matthew E; Woolner, Sarah et al. (2018) An interaction between myosin-10 and the cell cycle regulator Wee1 links spindle dynamics to mitotic progression in epithelia. J Cell Biol 217:849-859 |
| Varjabedian, Ani; Kita, Angela; Bement, William (2018) Living Xenopus oocytes, eggs, and embryos as models for cell division. Methods Cell Biol 144:259-285 |
| Breznau, Elaina B; Murt, Megan; Blasius, T Lynne et al. (2017) The MgcRacGAP SxIP motif tethers Centralspindlin to microtubule plus ends in Xenopus laevis. J Cell Sci 130:1809-1821 |
| Larson, Matthew E; Bement, William M (2017) Automated mitotic spindle tracking suggests a link between spindle dynamics, spindle orientation, and anaphase onset in epithelial cells. Mol Biol Cell 28:746-759 |
| Holmes, William R; Golding, Adriana E; Bement, William M et al. (2016) A mathematical model of GTPase pattern formation during single-cell wound repair. Interface Focus 6:20160032 |
| Severson, Aaron F; von Dassow, George; Bowerman, Bruce (2016) Oocyte Meiotic Spindle Assembly and Function. Curr Top Dev Biol 116:65-98 |
| Goryachev, Andrew B; Leda, Marcin; Miller, Ann L et al. (2016) How to make a static cytokinetic furrow out of traveling excitable waves. Small GTPases 7:65-70 |
| Davenport, Nicholas R; Bement, William M (2016) Cell repair: Revisiting the patch hypothesis. Commun Integr Biol 9:e1253643 |
| Davenport, Nicholas R; Sonnemann, Kevin J; Eliceiri, Kevin W et al. (2016) Membrane dynamics during cellular wound repair. Mol Biol Cell 27:2272-85 |
| Sandquist, Joshua C; Larson, Matthew E; Hine, Ken J (2016) Myosin-10 independently influences mitotic spindle structure and mitotic progression. Cytoskeleton (Hoboken) 73:351-64 |
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