Agency
National Institute of Health (NIH)
Institute
National Institute of General Medical Sciences (NIGMS)
Type
Research Project (R01)
Project #
1R01GM054221-01
Application #
2193612
Study Section
Bio-Organic and Natural Products Chemistry Study Section (BNP)
Project Start
1996-04-01
Project End
2000-03-31
Budget Start
1996-04-01
Budget End
1997-03-31
Support Year
1
Fiscal Year
1996
Total Cost
Indirect Cost
Name
University of Florida
Department
Pharmacology
Type
Schools of Medicine
DUNS #
073130411
City
Gainesville
State
FL
Country
United States
Zip Code
32611
Lanigan, Mark D; Kalman, Katalin; Lefievre, Yann et al. (2002) Mutating a critical lysine in ShK toxin alters its binding configuration in the pore-vestibule region of the voltage-gated potassium channel, Kv1.3. Biochemistry 41:11963-71
Fanger, C M; Rauer, H; Neben, A L et al. (2001) Calcium-activated potassium channels sustain calcium signaling in T lymphocytes. Selective blockers and manipulated channel expression levels. J Biol Chem 276:12249-56
Lanigan, M D; Pennington, M W; Lefievre, Y et al. (2001) Designed peptide analogues of the potassium channel blocker ShK toxin. Biochemistry 40:15528-37
Lanigan, M D; Tudor, J E; Pennington, M W et al. (2001) A helical capping motif in ShK toxin and its role in helix stabilization. Biopolymers 58:422-36
Rauer, H; Lanigan, M D; Pennington, M W et al. (2000) Structure-guided transformation of charybdotoxin yields an analog that selectively targets Ca(2+)-activated over voltage-gated K(+) channels. J Biol Chem 275:1201-8
Ghanshani, S; Wulff, H; Miller, M J et al. (2000) Up-regulation of the IKCa1 potassium channel during T-cell activation. Molecular mechanism and functional consequences. J Biol Chem 275:37137-49
Pennington, M W; Lanigan, M D; Kalman, K et al. (1999) Role of disulfide bonds in the structure and potassium channel blocking activity of ShK toxin. Biochemistry 38:14549-58
Rauer, H; Pennington, M; Cahalan, M et al. (1999) Structural conservation of the pores of calcium-activated and voltage-gated potassium channels determined by a sea anemone toxin. J Biol Chem 274:21885-92
Li, H L; Sui, H X; Ghanshani, S et al. (1998) Two-dimensional crystallization and projection structure of KcsA potassium channel. J Mol Biol 282:211-6
Tudor, J E; Pennington, M W; Norton, R S (1998) Ionisation behaviour and solution properties of the potassium-channel blocker ShK toxin. Eur J Biochem 251:133-41

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