The long term goal of this research is to determine the molecular events which underlie the transport of lysosomal proteins to the yeast lysosome-equivalent, the vacuole. This application focuses on a Ras-like GTPase, Vps21p, and a Sec1 homolog, Vps45p, shown to be important in vacuolar protein sorting. Dr. Horazdovsky will define precisely the step at which these proteins act, and begin to look for interacting proteins and their mechanisms of modulation of Vps21p.
Other aims i nclude analysis of transport vesicles carrying Golgi derived proteins to the vacuole, and characterization of the proteinase A sorting proteins.

Agency
National Institute of Health (NIH)
Institute
National Institute of General Medical Sciences (NIGMS)
Type
Research Project (R01)
Project #
5R01GM055301-04
Application #
6180661
Study Section
Cellular Biology and Physiology Subcommittee 1 (CBY)
Program Officer
Shapiro, Bert I
Project Start
1997-06-01
Project End
2002-05-31
Budget Start
2000-06-01
Budget End
2001-05-31
Support Year
4
Fiscal Year
2000
Total Cost
$196,695
Indirect Cost
Name
University of Texas Sw Medical Center Dallas
Department
Biochemistry
Type
Schools of Medicine
DUNS #
City
Dallas
State
TX
Country
United States
Zip Code
75390