The long term goal is to solve the long-standing question of how myosin functions as a motor. The principal investigator proposes to determine the biochemical defect in the myosins from cold sensitive mutants in order to uncover which part of the mechanical cycle is perturbed. He will then isolate intragenic suppressor mutations to these cold- sensitive mutants and determine the sites of reversion. He will then characterize these suppressor strains, and select some for further biochemical characterization. Finally. he will characterize biochemical myosins carrying the suppressor mutations after separation from the original cold-sensitive mutations. The principal investigator plans to confirm, modify or replace models for the various conformations that myosin assumes during its mechanical cycle.

Agency
National Institute of Health (NIH)
Institute
National Institute of General Medical Sciences (NIGMS)
Type
Research Project (R01)
Project #
5R01GM055977-03
Application #
2750143
Study Section
Biological Sciences 2 (BIOL)
Project Start
1996-08-01
Project End
2000-07-31
Budget Start
1998-08-01
Budget End
1999-07-31
Support Year
3
Fiscal Year
1998
Total Cost
Indirect Cost
Name
University of Arizona
Department
Microbiology/Immun/Virology
Type
Schools of Arts and Sciences
DUNS #
City
Tucson
State
AZ
Country
United States
Zip Code
85721