): The broad objective of this application is to determine the developmental relationships among populations of cells in the hindbrain and the craniofacial tissues of mammals. The development these tissues are inextricably linked to one another by the migration of neural crest cells from the hindbrain to form much of the skeletal and connective tissue elements of the head and neck. In this manner some of the positional information encoded by restricted patterns of gene expression in the hindbrain and which guides its development, is also projected onto nascent craniofacial tissues. It is generally agreed that members of the Hox gene family contribute to the genetic specification of hindbrain and craniofacial tissues. Here we propose to use a novel lineage marking paradigm to determine the fates of cells that express specific Hox genes, both in the context of the normal animal and in mice in which the function of the Hox gene is altered. In some cases the alteration will include the complete elimination of gene function. We will also examine the alterations in Hox gene function occasioned by the elimination of individual members of the Meis gene family, which are known to collaborate with Hox genes in the patterning of appendages, and which may well also contribute to the patterning of the hindbrain and craniofacial tissues.

Agency
National Institute of Health (NIH)
Institute
National Institute of General Medical Sciences (NIGMS)
Type
Research Project (R01)
Project #
5R01GM056525-11
Application #
6603442
Study Section
Cell Development and Function Integrated Review Group (CDF)
Program Officer
Greenberg, Judith H
Project Start
1993-05-01
Project End
2005-06-30
Budget Start
2003-07-01
Budget End
2004-06-30
Support Year
11
Fiscal Year
2003
Total Cost
$334,348
Indirect Cost
Name
Case Western Reserve University
Department
Neurosciences
Type
Schools of Medicine
DUNS #
077758407
City
Cleveland
State
OH
Country
United States
Zip Code
44106
Rottkamp, Catherine A; Lobur, Katherine J; Wladyka, Cynthia L et al. (2008) Pbx3 is required for normal locomotion and dorsal horn development. Dev Biol 314:23-39
O'Gorman, Stephen (2005) Second branchial arch lineages of the middle ear of wild-type and Hoxa2 mutant mice. Dev Dyn 234:124-31
Guo, Sun-Wei (2002) Sibling recurrence risk ratio as a measure of genetic effect: caveat emptor! Am J Hum Genet 70:818-9