Abstract

The yeast genome sequence is now known, a major technical accomplishment that offer unprecedented opportunities for study of the cell biology of the simplest eukaryote. Knowledge of the interactions of regulatory and structural proteins with DNA in vivo is important for application-of the yeast DNA sequence to its function. This proposal addresses how to accomplish that goal. It builds on emerging applications of DNA methyltransferases to map protein-DNA interactions in living cells by developing methodology that will allow resolution (

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Institute of General Medical Sciences (NIGMS)
Type
Research Project (R01)
Project #
5R01GM056907-03
Application #
2910375
Study Section
Special Emphasis Panel (ZHG1-HGR-N (J1))
Project Start
1997-05-01
Project End
2001-04-30
Budget Start
1999-05-01
Budget End
2001-04-30
Support Year
3
Fiscal Year
1999
Total Cost
Indirect Cost

  Institution

Name
Pennsylvania State University
Department
Biochemistry
Type
Schools of Arts and Sciences
DUNS #
City
University Park
State
PA
Country
United States
Zip Code
16802

  Publications

Kladde, M P; Xu, M; Simpson, R T (1999) DNA methyltransferases as probes of chromatin structure in vivo. Methods Enzymol 304:431-47