This proposal concerns Kip3, a new kinesin identified from the yeast database which has been implicated in nuclear orientation prior to anaphase. Localization of Kip3 to astral tubules has been demonstrated. Extensive microscopy is planned to test the hypothesis that it is these astral tubules that serve to correctly orient the spindle. A variety of genetic screens are planned to find the proteins that may function to couple Kip3 to the cell cortex and thereby allow it to correctly orient the nucleus through its action on astral tubules. The interaction with tubules and with associated proteins will be explored biochemically.

Agency
National Institute of Health (NIH)
Institute
National Institute of General Medical Sciences (NIGMS)
Type
Research Project (R01)
Project #
5R01GM057441-04
Application #
6386873
Study Section
Cellular Biology and Physiology Subcommittee 1 (CBY)
Program Officer
Deatherage, James F
Project Start
1998-04-01
Project End
2003-03-31
Budget Start
2001-04-01
Budget End
2002-03-31
Support Year
4
Fiscal Year
2001
Total Cost
$208,196
Indirect Cost
Name
University of Pennsylvania
Department
Veterinary Sciences
Type
Schools of Veterinary Medicine
DUNS #
042250712
City
Philadelphia
State
PA
Country
United States
Zip Code
19104