This FIRST award proposal is to examine the activation of baseline K+ leak channels by volatile anesthetics.
Specific aims are (1) to determine the mechanism of channel activation by examining how volatile anesthetics in a range of concentrations alter channel properties, in addition to study the basic physiology and pharmacology of this channel type; (2), to identify and characterize the binding sites at which volatile anesthetics act by examining their ability to occlude actions of endogenous messengers such as arachidonic acid and the ability of halogenated non-anesthetics to act on the channels and to inhibit the actions of isoflurane, and (3) to determine the role of these channels in modulating neuronal excitability and synaptic transmission. Studies will be carried out in cerebellar granule neurons using whole cell ruptured patch and excised patch techniques on neurons in culture and in thin slices.

Agency
National Institute of Health (NIH)
Institute
National Institute of General Medical Sciences (NIGMS)
Type
Research Project (R01)
Project #
5R01GM057529-05
Application #
6519882
Study Section
Surgery, Anesthesiology and Trauma Study Section (SAT)
Program Officer
Cole, Alison E
Project Start
1998-05-01
Project End
2004-04-30
Budget Start
2002-05-01
Budget End
2004-04-30
Support Year
5
Fiscal Year
2002
Total Cost
$152,425
Indirect Cost
Name
University of California San Francisco
Department
Anesthesiology
Type
Schools of Medicine
DUNS #
073133571
City
San Francisco
State
CA
Country
United States
Zip Code
94143