This revised proposal is focused on three complementary specific aims: (1) development of an E. coli strain capable of supporting shikimic acid biosynthesis efficiently, while not resorting to the PTS system for sugar transport (which has poor atom economy), (2) Evaluate the pros and cons of engineering shikimic acid biosynthesis in S. cerevisiae and G. oxydans, neither of which rely on the PTS system for glucose transport, and (3) develop strategies for further buttressing shikimic acid biosynthesis by exogenous addition of simple feedstocks such as TCA cycle intermediates to cell cultures.

Agency
National Institute of Health (NIH)
Institute
National Institute of General Medical Sciences (NIGMS)
Type
Research Project (R01)
Project #
5R01GM058684-03
Application #
6386381
Study Section
Bio-Organic and Natural Products Chemistry Study Section (BNP)
Program Officer
Jones, Warren
Project Start
1999-08-01
Project End
2003-07-31
Budget Start
2001-08-01
Budget End
2002-07-31
Support Year
3
Fiscal Year
2001
Total Cost
$209,284
Indirect Cost
Name
Michigan State University
Department
Chemistry
Type
Schools of Arts and Sciences
DUNS #
193247145
City
East Lansing
State
MI
Country
United States
Zip Code
48824