An important problem in developmental biology is how positional information created by threshold responses to diffusible signals is translated into the formation of specialized morphological structures at different locations. The initiation of wing vein development in Drosophila provides a particularly favorable system for investigating this link between patterning and formation of adult structures. A great deal is already known about how positions are specified along the primary axes of the wing and how this information leads to initiation of vein-specific patterns of gene expression in different vein primordia. The overall goal of this grant proposal is to understand the mechanism by which positional information is converted into vein specific differentiation programs for the second and fifth longitudinal wing veins, which form at opposite positions along the anterior-posterior axis of the wing. Previous analysis revealed that veins form in narrow lines along boundaries between abutting domains of cells. Vein primordia form along the edge of one domain of cells in response to diffusible short range signals emanating from adjacent cells that are unable to respond to the vein inducing signal they produce. A central aim in understanding this """"""""for-export-only"""""""" mode of signaling is to determine the molecular identity of the putative vein inductive signals. In addition to analyzing the mechanisms by which vein morphogenesis is initiated, we will also compare the activities of the knirps(kni) and abrupt (ab) genes, which play key roles in organizing vein specific gene expression programs in the second and fifth vein primordia respectively. The proposed experiments are relevant to human health in two ways. First, the problem of forming lines during development is a general process. Consequently, insights gained from the proposed experiments will be applicable to many vertebrate stages of development. Second, defects in several genes involved in determining the positions of veins cause birth defects or cancer when mutated in humans. Thus, knowledge gained from these studies should have broad applications to understanding vertebrate development as well as offering possible insights into mechanisms of human disease.
| Iyadurai, Stanley J P; Robinson, John T; Ma, Lingzhi et al. (2008) Dynein and Star interact in EGFR signaling and ligand trafficking. J Cell Sci 121:2643-51 |
| Reiter, Lawrence T; Seagroves, Tiffany N; Bowers, Megan et al. (2006) Expression of the Rho-GEF Pbl/ECT2 is regulated by the UBE3A E3 ubiquitin ligase. Hum Mol Genet 15:2825-35 |
| Cook, Orna; Biehs, Brian; Bier, Ethan (2004) brinker and optomotor-blind act coordinately to initiate development of the L5 wing vein primordium in Drosophila. Development 131:2113-24 |
| Lunde, Karen; Trimble, Jennifer L; Guichard, Annabel et al. (2003) Activation of the knirps locus links patterning to morphogenesis of the second wing vein in Drosophila. Development 130:235-48 |
| Reiter, L T; Potocki, L; Chien, S et al. (2001) A systematic analysis of human disease-associated gene sequences in Drosophila melanogaster. Genome Res 11:1114-25 |
