The two principal investigators in this collaboration propose to study the evolution of a bacterium from a commensal to a pathogen through selection operating on pre-existing alleles, rather than through the acquisition of substantially different genes through horizontal transfer. (An example of the latter phenomenon is the """"""""pathogenicity island"""""""" or a bacteriophage with a toxin gene.) The investigators use the term """"""""pathoadaption"""""""" for the process of selection of allele variants that """"""""promote adaptation of the bacterial pathogens to a particular mode of pathogenesis."""""""" They propose to use for a model system type 1 fimbriae and adhesins of Escherichia coli and in particular the evolution of the adhesins for adaptation to the urinary tract and upper respiratory tract. For the study, the investigators will gather together representative strains and further characterize the fimbrial genes and the control regions for the genes. They will use Single Stranded Conformational Polymorphism and sequencing to distinguish the variants and to identify """"""""signatures"""""""" for adaptation to the human host microenvironment in a survey type of study. In a prospective study they will use an animal model to test their hypothesis that variant alleles of fimbrial genes that promote persistence in the animal urinary tract will be selected and then detected in the population.

Agency
National Institute of Health (NIH)
Institute
National Institute of General Medical Sciences (NIGMS)
Type
Research Project (R01)
Project #
5R01GM060731-02
Application #
6351349
Study Section
Special Emphasis Panel (ZRG1-GEN (01))
Program Officer
Eckstrand, Irene A
Project Start
2000-02-01
Project End
2004-01-31
Budget Start
2001-02-01
Budget End
2002-01-31
Support Year
2
Fiscal Year
2001
Total Cost
$236,217
Indirect Cost
Name
State University New York Stony Brook
Department
Biology
Type
Schools of Arts and Sciences
DUNS #
804878247
City
Stony Brook
State
NY
Country
United States
Zip Code
11794
Monzón, J; Kays, R; Dykhuizen, D E (2014) Assessment of coyote-wolf-dog admixture using ancestry-informative diagnostic SNPs. Mol Ecol 23:182-97
Gray, Sarah M; Akob, Denise M; Green, Stefan J et al. (2012) The bacterial composition within the Sarracenia purpurea model system: local scale differences and the relationship with the other members of the food web. PLoS One 7:e50969
Brisson, Dustin; Vandermause, Mary F; Meece, Jennifer K et al. (2010) Evolution of northeastern and midwestern Borrelia burgdorferi, United States. Emerg Infect Dis 16:911-7
Stahlhut, Steen G; Chattopadhyay, Sujay; Struve, Carsten et al. (2009) Population variability of the FimH type 1 fimbrial adhesin in Klebsiella pneumoniae. J Bacteriol 191:1941-50
Stahlhut, Steen G; Tchesnokova, Veronika; Struve, Carsten et al. (2009) Comparative structure-function analysis of mannose-specific FimH adhesins from Klebsiella pneumoniae and Escherichia coli. J Bacteriol 191:6592-601
Chattopadhyay, Sujay; Weissman, Scott J; Minin, Vladimir N et al. (2009) High frequency of hotspot mutations in core genes of Escherichia coli due to short-term positive selection. Proc Natl Acad Sci U S A 106:12412-7
Chattopadhyay, Sujay; Paranjpye, Rohinee N; Dykhuizen, Daniel E et al. (2009) Comparative evolutionary analysis of the major structural subunit of Vibrio vulnificus type IV pili. Mol Biol Evol 26:2185-96
Dykhuizen, Daniel E; Brisson, Dustin; Sandigursky, Sabina et al. (2008) The propensity of different Borrelia burgdorferi sensu stricto genotypes to cause disseminated infections in humans. Am J Trop Med Hyg 78:806-10
Stoebel, Daniel M; Dean, Antony M; Dykhuizen, Daniel E (2008) The cost of expression of Escherichia coli lac operon proteins is in the process, not in the products. Genetics 178:1653-60
Brisson, Dustin; Dykhuizen, Daniel E; Ostfeld, Richard S (2008) Conspicuous impacts of inconspicuous hosts on the Lyme disease epidemic. Proc Biol Sci 275:227-35

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