Abstract

application) This revised application aims to apply Dr. Caffrey's expertise in lipids to the rational design of cubic lipid phases for the crystallization of membrane proteins. Though a number of membrane proteins, notably bacteriorhodopsin (BR), have been successfully crystallized from lipid cubic mesophases, there has been no careful study of the optimum state of the mesophase, and of the details of the boundary between the growing crystal and the bulk phase. Instead only empirical procedures are used to obtain crystals. To remedy this, Dr. Caffrey proposes to extend his library of phase diagrams for monoacylglycerols, which form cubic mesophases, and to study the relationship between the phase diagrams and the optimum crystallization of BR and of a bacterial photoreaction center, RC. The results of these studies, together with an analysis in detail of the crystal/lipid interface will then be used in attempts at rational crystallization of the previously uncrystallized photosystem II from Chlamydomonas.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Institute of General Medical Sciences (NIGMS)
Type
Research Project (R01)
Project #
1R01GM061070-01A1
Application #
6285859
Study Section
Biophysical Chemistry Study Section (BBCB)
Program Officer
Chin, Jean
Project Start
2001-01-01
Project End
2004-12-31
Budget Start
2001-01-01
Budget End
2001-12-31
Support Year
1
Fiscal Year
2001
Total Cost
$242,856
Indirect Cost

  Institution

Name
Ohio State University
Department
Chemistry
Type
Schools of Arts and Sciences
DUNS #
098987217
City
Columbus
State
OH
Country
United States
Zip Code
43210

  Publications

Lyons, Joseph A; Aragão, David; Slattery, Orla et al. (2012) Structural insights into electron transfer in caa3-type cytochrome oxidase. Nature 487:514-8
Caffrey, Martin; Cherezov, Vadim (2009) Crystallizing membrane proteins using lipidic mesophases. Nat Protoc 4:706-31
Cherezov, Vadim; Hofer, Nicole; Szebenyi, Doletha M E et al. (2008) Insights into the mode of action of a putative zinc transporter CzrB in Thermus thermophilus. Structure 16:1378-88
Cherezov, Vadim; Liu, Wei; Derrick, Jeremy P et al. (2008) In meso crystal structure and docking simulations suggest an alternative proteoglycan binding site in the OpcA outer membrane adhesin. Proteins 71:24-34
Hofer, Nicole; Kolaj, Olga; Li, Hui et al. (2007) Crystallization and preliminary X-ray diffraction analysis of a soluble domain of the putative zinc transporter CzrB from Thermus thermophilus. Acta Crystallogr Sect F Struct Biol Cryst Commun 63:673-7
Boyle-Roden, E; Hoefer, N; Dey, K K et al. (2007) High resolution 1H NMR of a lipid cubic phase using a solution NMR probe. J Magn Reson 189:13-9
Luan, Binquan; Caffrey, Martin; Aksimentiev, Aleksei (2007) Structure refinement of the OpcA adhesin using molecular dynamics. Biophys J 93:3058-69
Cherezov, Vadim; Caffrey, Martin (2007) Membrane protein crystallization in lipidic mesophases. A mechanism study using X-ray microdiffraction. Faraday Discuss 136:195-212;discussion 213-29
Cherezov, Vadim; Clogston, Jeffrey; Papiz, Miroslav Z et al. (2006) Room to move: crystallizing membrane proteins in swollen lipidic mesophases. J Mol Biol 357:1605-18
Siegel, D P; Cherezov, V; Greathouse, D V et al. (2006) Transmembrane peptides stabilize inverted cubic phases in a biphasic length-dependent manner: implications for protein-induced membrane fusion. Biophys J 90:200-11

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