The long-term objective of the proposed study is to establish how molecular variation in specific proteins involved in a signal transduction pathway contributes to phenotypic variation. The project focuses on the developmental pathways associated with wing shape and photoreceptor variation in Drosophila. The general approach is to use genetic """"""""tester"""""""" backgrounds that bear selected hypo- and hypermorphic mutations shown by Dr. Gibson to reveal modifiers of expression. He proposes that these modifiers are genes in the same signal transduction pathway that contribute to potential subtle perhaps cryptic standing genetic variation in natural populations. In these """"""""sensitized"""""""" backgrounds measured phenotypic effects are mapped to the candidate genes of the associated EGF-Ras pathway using association or linkage disequilibrium mapping, as has been used to define QTLs for bristle variation in natural populations of D. melanogaster. The objective at this point is not to identify specific mutations responsible for functional variation yet, but to quantify relative effects of different loci in the pathway.

Agency
National Institute of Health (NIH)
Institute
National Institute of General Medical Sciences (NIGMS)
Type
Research Project (R01)
Project #
5R01GM061600-02
Application #
6387194
Study Section
Genetics Study Section (GEN)
Program Officer
Eckstrand, Irene A
Project Start
2000-08-01
Project End
2004-07-31
Budget Start
2001-08-01
Budget End
2002-07-31
Support Year
2
Fiscal Year
2001
Total Cost
$183,125
Indirect Cost
Name
North Carolina State University Raleigh
Department
Genetics
Type
Schools of Earth Sciences/Natur
DUNS #
City
Raleigh
State
NC
Country
United States
Zip Code
27695
Williams, Stephanie; Dew-Budd, Kelly; Davis, Kristen et al. (2015) Metabolomic and Gene Expression Profiles Exhibit Modular Genetic and Dietary Structure Linking Metabolic Syndrome Phenotypes in Drosophila. G3 (Bethesda) 5:2817-29
Reed, Laura K; Lee, Kevin; Zhang, Zhi et al. (2014) Systems genomics of metabolic phenotypes in wild-type Drosophila melanogaster. Genetics 197:781-93
Reed, Laura K; Williams, Stephanie; Springston, Mastafa et al. (2010) Genotype-by-diet interactions drive metabolic phenotype variation in Drosophila melanogaster. Genetics 185:1009-19
Idaghdour, Youssef; Czika, Wendy; Shianna, Kevin V et al. (2010) Geographical genomics of human leukocyte gene expression variation in southern Morocco. Nat Genet 42:62-7
Goering, Lisa M; Hunt, Priscilla K; Heighington, Cassandra et al. (2009) Association of orthodenticle with natural variation for early embryonic patterning in Drosophila melanogaster. J Exp Zool B Mol Dev Evol 312:841-54
Kennerly, Erin M; Idaghdour, Youssef; Olby, Natasha J et al. (2009) Pharmacogenetic association study of 30 genes with phenobarbital drug response in epileptic dogs. Pharmacogenet Genomics 19:911-22
Kennerly, Erin; Ballmann, Anne; Martin, Stanton et al. (2008) A gene expression signature of confinement in peripheral blood of red wolves (Canis rufus). Mol Ecol 17:2782-91
Moser, Jennifer M; Carbone, Ignazio; Arasu, Prema et al. (2007) Impact of population structure on genetic diversity of a potential vaccine target in the canine hookworm (Ancylostoma caninum). J Parasitol 93:796-805
Goering, Lisa M; Gibson, Greg (2005) Genetic variation for dorsal-ventral patterning of the Drosophila melanogaster eggshell. Evol Dev 7:81-8
Dworkin, Ian; Palsson, Arnar; Gibson, Greg (2005) Replication of an Egfr-wing shape association in a wild-caught cohort of Drosophila melanogaster. Genetics 169:2115-25

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