Cell cycle controls are organized into a loop, so that cycles may occur over and over again. These controls are of several kinds: transcriptional controls, proteolytic controls, protein kinase controls, etc. The development of microarray technology has now made it far easier and more efficient to investigate all kinds of transcriptional controls. Indeed, microarray studies on the transcriptional regulation of the cell cycle have provided a wealth of data. In this proposal, microarray technology will be used to continue the study of the transcriptional control of the yeast cell cycle. In particular, an overview of the circular nature of cell cycle controls will be sought.
In Aim 1, we will complete the task of assigning cell cycle regulated genes to their regulating transcription factors.
In Aim 2, we will find out how the time at which cell cycle genes are expressed is determined, and we will define the extent and mechanisms of combinatorial control in the cycle.
In Aim 3, we will test the hypothesis that an oscillation in the state of histone acetylation is a major mechanism of cell cycle transcriptional control. ? ?

Agency
National Institute of Health (NIH)
Institute
National Institute of General Medical Sciences (NIGMS)
Type
Research Project (R01)
Project #
5R01GM064813-07
Application #
7243420
Study Section
Cell Development and Function Integrated Review Group (CDF)
Program Officer
Zatz, Marion M
Project Start
2001-05-01
Project End
2009-05-31
Budget Start
2007-06-01
Budget End
2008-05-31
Support Year
7
Fiscal Year
2007
Total Cost
$330,679
Indirect Cost
Name
State University New York Stony Brook
Department
Genetics
Type
Schools of Medicine
DUNS #
804878247
City
Stony Brook
State
NY
Country
United States
Zip Code
11794
Di Talia, Stefano; Wang, Hongyin; Skotheim, Jan M et al. (2009) Daughter-specific transcription factors regulate cell size control in budding yeast. PLoS Biol 7:e1000221
Pyne, Saumyadipta; Gutman, Roee; Kim, Chang Sik et al. (2009) Phase Coupled Meta-analysis: sensitive detection of oscillations in cell cycle gene expression, as applied to fission yeast. BMC Genomics 10:440
Wan, Lihong; Niu, Hengyao; Futcher, Bruce et al. (2008) Cdc28-Clb5 (CDK-S) and Cdc7-Dbf4 (DDK) collaborate to initiate meiotic recombination in yeast. Genes Dev 22:386-97
Lo, Hsiao-Chi; Wan, Lihong; Rosebrock, Adam et al. (2008) Cdc7-Dbf4 regulates NDT80 transcription as well as reductional segregation during budding yeast meiosis. Mol Biol Cell 19:4956-67
Pyne, Saumyadipta; Futcher, Bruce; Skiena, Steve (2006) Meta-analysis based on control of false discovery rate: combining yeast ChIP-chip datasets. Bioinformatics 22:2516-22
Oliva, Anna; Rosebrock, Adam; Ferrezuelo, Francisco et al. (2005) The cell cycle-regulated genes of Schizosaccharomyces pombe. PLoS Biol 3:e225