Agency
National Institute of Health (NIH)
Institute
National Institute of General Medical Sciences (NIGMS)
Type
Research Project (R01)
Project #
5R01GM065406-02
Application #
6693410
Study Section
Bio-Organic and Natural Products Chemistry Study Section (BNP)
Program Officer
Lograsso, Philip
Project Start
2003-01-01
Project End
2007-12-31
Budget Start
2004-01-01
Budget End
2004-12-31
Support Year
2
Fiscal Year
2004
Total Cost
$246,358
Indirect Cost
Name
University of Wisconsin Madison
Department
Chemistry
Type
Schools of Arts and Sciences
DUNS #
161202122
City
Madison
State
WI
Country
United States
Zip Code
53715
Krusemark, Casey J; Frey, Brian L; Belshaw, Peter J et al. (2009) Modifying the charge state distribution of proteins in electrospray ionization mass spectrometry by chemical derivatization. J Am Soc Mass Spectrom 20:1617-25
Miller, Russell A (2009) Ligand-regulated peptide aptamers. Methods Mol Biol 535:315-31
Krusemark, Casey J; Ferguson, Jonathan T; Wenger, Craig D et al. (2008) Global amine and acid functional group modification of proteins. Anal Chem 80:713-20
Frey, Brian L; Krusemark, Casey J; Ledvina, Aaron R et al. (2008) Ion-Ion Reactions with Fixed-Charge Modified Proteins to Produce Ions in a Single, Very High Charge State. Int J Mass Spectrom 276:136-143
Lamos, Shane M; Shortreed, Michael R; Frey, Brian L et al. (2007) Relative quantification of carboxylic acid metabolites by liquid chromatography-mass spectrometry using isotopic variants of cholamine. Anal Chem 79:5143-9
Krusemark, Casey J; Belshaw, Peter J (2007) Covalent labelling of fusion proteins in live cells via an engineered receptor-ligand pair. Org Biomol Chem 5:2201-4
Miller, Russell A; Binkowski, Brock F; Belshaw, Peter J (2007) Ligand-regulated peptide aptamers that inhibit the 5'-AMP-activated protein kinase. J Mol Biol 365:945-57
Lamos, Shane M; Krusemark, Casey J; McGee, Christopher J et al. (2006) Mixed isotope photoaffinity reagents for identification of small-molecule targets by mass spectrometry. Angew Chem Int Ed Engl 45:4329-33
Shortreed, Michael R; Lamos, Shane M; Frey, Brian L et al. (2006) Ionizable isotopic labeling reagent for relative quantification of amine metabolites by mass spectrometry. Anal Chem 78:6398-403
Levitsky, Konstantin; Boersma, Melissa D; Ciolli, Christopher J et al. (2005) Exo-mechanism proximity-accelerated alkylations: investigations of linkers, electrophiles and surface mutations in engineered cyclophilin-cyclosporin systems. Chembiochem 6:890-9

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