Our long-term goals are to understand the spatial and temporal events involved in gene expression/silencing in living cells. Understanding the dynamics of these events in vivo will provide the basis to identify changes that occur in cells or tissues associated with various disease states. We propose to utilize a live cell system to allow us to visualize a fundamental cellular process, gene silencing, at the levels of DNA, RNA, and protein, directly in living cells. This system will be used to assess the assembly and dynamics of Polycomb group proteins at a regulatable genetic locus. Using this system we will characterize the temporal pattern of factor arrival and release, and correlate it to changes in chromatin structure. In addition, we will study the dynamics of this specific genetic locus during the transition from the """"""""on"""""""" to the """"""""off"""""""" transcriptional state, in relation to RNA clearance and nuclear organization. The development and utilization of this live cell system will allow us to gain important insight into the spatial and temporal aspects of gene silencing that cannot be obtained by previously used approaches. ? ?

Agency
National Institute of Health (NIH)
Institute
National Institute of General Medical Sciences (NIGMS)
Type
Research Project (R01)
Project #
5R01GM071407-04
Application #
7274755
Study Section
Special Emphasis Panel (ZRG1-SSS-U (04))
Program Officer
Carter, Anthony D
Project Start
2004-09-03
Project End
2009-08-31
Budget Start
2007-09-01
Budget End
2009-08-31
Support Year
4
Fiscal Year
2007
Total Cost
$310,885
Indirect Cost
Name
Cold Spring Harbor Laboratory
Department
Type
DUNS #
065968786
City
Cold Spring Harbor
State
NY
Country
United States
Zip Code
11724
Zhao, Rui; Bodnar, Megan S; Spector, David L (2009) Nuclear neighborhoods and gene expression. Curr Opin Genet Dev 19:172-9