Aerobic organisms, from bacteria to plants and animals, have evolved sensing, signaling, and protective mechanisms to cope with various reactive oxygen species (ROS) that are inevitably generated both intra- and extracellularly. Elucidation of these mechanisms is a problem of broad biological significance. Although cellular responses to ROS such as hydrogen peroxide and superoxide have been extensively studied, relatively little is known about biological responses to singlet oxygen, an especially toxic type of ROS that causes oxidative damage to numerous critical cellular components, such as unsaturated lipids, proteins, and DNA. Singlet oxygen is commonly generated by photosensitization reactions that are the basis for the photooxidative tissue damage observed in humans suffering from porphyria and possibly age-related macular degeneration. On the other hand, photosensitization has been exploited in strategies for photodynamic therapy of certain human cancers. This proposal aims to address the relative deficiency of knowledge about singlet oxygen signaling by using molecular genetic and genomic approaches to dissect the singlet oxygen acclimation response in a unicelluar green alga, Chlamydomonas reinhardtii, a model photosynthetic eukaryote. Like other oxygenic phototrophs, Chlamydomonas must cope with high endogenous concentrations of both oxygen and chlorophyll, a potent photosensitizer and may therefore have evolved particularly robust mechanisms for perceiving and responding to singlet oxygen.
The specific aims of this proposal are (1) to perform physiological and biochemical characterization of the singlet oxygen acclimation response in Chlamydomonas, (2) to use whole-genome microarray analysis to identify the set of genes whose expression changes during acclimation to singlet oxygen, (3) to use reverse genetics approaches to test the necessity of specific candidate genes in singlet oxygen acclimation, and (4) to perform a forward genetic dissection of singlet oxygen acclimation, including (5) the isolation of a gene that is defective in an already identified acclimation mutant, called sos1. This investigation will help illuminate studies of singlet oxygen biology in other organisms and provide fundamental knowledge about how eukaryotic cells sense and respond to singlet oxygen.

Agency
National Institute of Health (NIH)
Institute
National Institute of General Medical Sciences (NIGMS)
Type
Research Project (R01)
Project #
5R01GM071908-02
Application #
6926214
Study Section
Cell Development and Function Integrated Review Group (CDF)
Program Officer
Anderson, James J
Project Start
2004-08-01
Project End
2008-07-31
Budget Start
2005-08-01
Budget End
2006-07-31
Support Year
2
Fiscal Year
2005
Total Cost
$264,240
Indirect Cost
Name
University of California Berkeley
Department
Other Basic Sciences
Type
Schools of Earth Sciences/Natur
DUNS #
124726725
City
Berkeley
State
CA
Country
United States
Zip Code
94704
Wakao, Setsuko; Chin, Brian L; Ledford, Heidi K et al. (2014) Phosphoprotein SAK1 is a regulator of acclimation to singlet oxygen in Chlamydomonas reinhardtii. Elife 3:e02286
Fischer, Beat B; Ledford, Heidi K; Wakao, Setsuko et al. (2012) SINGLET OXYGEN RESISTANT 1 links reactive electrophile signaling to singlet oxygen acclimation in Chlamydomonas reinhardtii. Proc Natl Acad Sci U S A 109:E1302-11
Fischer, Beat B; Eggen, Rik Il; Niyogi, Krishna K (2010) Characterization of singlet oxygen-accumulating mutants isolated in a screen for altered oxidative stress response in Chlamydomonas reinhardtii. BMC Plant Biol 10:279
Li, Zhirong; Wakao, Setsuko; Fischer, Beat B et al. (2009) Sensing and responding to excess light. Annu Rev Plant Biol 60:239-60
Gutman, Benjamin L; Niyogi, Krishna K (2009) Evidence for base excision repair of oxidative DNA damage in chloroplasts of Arabidopsis thaliana. J Biol Chem 284:17006-12
Ledford, Heidi K; Chin, Brian L; Niyogi, Krishna K (2007) Acclimation to singlet oxygen stress in Chlamydomonas reinhardtii. Eukaryot Cell 6:919-30