In flies, as in many other animals, mating does not guarantee successful reproduction. Postmating prefertilization interactions between males and females (e.g., sperm competition, sperm storage by females) also play a central role in reproduction. Moreover, post-copulatory interactions may have broad evolutionary implications. For example, postmating sexual selection may contribute to the evolution of reproductive isolation. Most research on post-copulatory pre-fertilization biology in flies has focused on male-expressed proteins such as seminal fluid proteins. Though it is well known that female flies are not simply passive vessels and that female-mediated interactions with male products are important to reproductive success, little is known about the genetics or population genetics of female postcopulatory biology. The proposed research aims to begin filling this basic gap in our understanding of Drosophila females.
The specific aims of the proposed work are to i) use molecular approaches to identify candidate genes potentially affecting female postcopulatory phenotypes in D. melanogaster, ii) use QTL analysis of recombinant inbred lines of D. melanogaster to identify regions of the genome harboring natural variants affecting female post-copulatory phenotypes, iii) use molecular population genetics to test hypotheses on the causes of variation in candidate genes, iv) investigate divergence of female postcopulatory phenotypes and evolution of postcopulatory incompatibilities in the melanogaster group.

National Institute of Health (NIH)
National Institute of General Medical Sciences (NIGMS)
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Special Emphasis Panel (ZRG1-GVE (01))
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Eckstrand, Irene A
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University of California Davis
Schools of Medicine
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Kolaczkowski, Bryan; Kern, Andrew D; Holloway, Alisha K et al. (2011) Genomic differentiation between temperate and tropical Australian populations of Drosophila melanogaster. Genetics 187:245-60
Holloway, Alisha K; Begun, David J; Siepel, Adam et al. (2008) Accelerated sequence divergence of conserved genomic elements in Drosophila melanogaster. Genome Res 18:1592-601
Li, Yong Fuga; Costello, James C; Holloway, Alisha K et al. (2008) ""Reverse ecology"" and the power of population genomics. Evolution 62:2984-94
Levine, Mia T; Begun, David J (2008) Evidence of spatially varying selection acting on four chromatin-remodeling loci in Drosophila melanogaster. Genetics 179:475-85
Turner, Thomas L; Levine, Mia T; Eckert, Melissa L et al. (2008) Genomic analysis of adaptive differentiation in Drosophila melanogaster. Genetics 179:455-73
Kern, Andrew D; Begun, David J (2008) Recurrent deletion and gene presence/absence polymorphism: telomere dynamics dominate evolution at the tip of 3L in Drosophila melanogaster and D. simulans. Genetics 179:1021-7
Lawniczak, Mara K N; Holloway, Alisha K; Begun, David J et al. (2008) Genomic analysis of the relationship between gene expression variation and DNA polymorphism in Drosophila simulans. Genome Biol 9:R125
Lawniczak, Mara K N; Begun, David J (2007) Molecular population genetics of female-expressed mating-induced serine proteases in Drosophila melanogaster. Mol Biol Evol 24:1944-51
Begun, David J; Lindfors, Heather A; Kern, Andrew D et al. (2007) Evidence for de novo evolution of testis-expressed genes in the Drosophila yakuba/Drosophila erecta clade. Genetics 176:1131-7
Levine, Mia T; Begun, David J (2007) Comparative population genetics of the immunity gene, Relish: is adaptive evolution idiosyncratic? PLoS One 2:e442

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