A primary objective of population genetics is to determine the nature of genetic variation and how it is maintained in natural populations. Mimulus guttatus is a wildflower that exhibits substantial genetic variation in flower size, a trait that has evolved extensively within the species complex. We will map the genetic loci responsible for variation in flower size within a single natural population of M. guttatus and determine the magnitude and distribution of allelic effects on a range of plant characters. The experiments will estimate the extent and pattern of dominance at QTL and the pleiotropic effects associated with flower size alleles. A streamlined breeding scheme will produce Nearly Isogenic Lines (NILs), necessary for positional cloning of the actual loci defining important QTL. They will also be used in field and lab studies to measure the direct fitness consequences of flower size variation at the scale of individual QTL. These data will be used to test hypotheses regarding the selective maintenance of flower size variation. Combined with results from previous and ongoing studies, these experiments will provide a detailed comparison of inter- and intra- specific variation in floral morphology within the M. guttatus species complex. Positional cloning of important floral QTLs will allow molecular population genetic studies of ecologically important genes. The proposed research will have broader impacts thorough both education and the development of scientific resources.

Agency
National Institute of Health (NIH)
Institute
National Institute of General Medical Sciences (NIGMS)
Type
Research Project (R01)
Project #
5R01GM073990-04
Application #
7617119
Study Section
Genetic Variation and Evolution Study Section (GVE)
Program Officer
Eckstrand, Irene A
Project Start
2006-05-01
Project End
2011-04-30
Budget Start
2009-05-01
Budget End
2011-04-30
Support Year
4
Fiscal Year
2009
Total Cost
$332,677
Indirect Cost
Name
University of Kansas Lawrence
Department
Biology
Type
Schools of Arts and Sciences
DUNS #
076248616
City
Lawrence
State
KS
Country
United States
Zip Code
66045
Brown, K E; Kelly, J K (2018) Antagonistic pleiotropy can maintain fitness variation in annual plants. J Evol Biol 31:46-56
Wessinger, Carolyn A; Kelly, John K; Jiang, Peng et al. (2018) SNP-skimming: A fast approach to map loci generating quantitative variation in natural populations. Mol Ecol Resour 18:1402-1414
Troth, Ashley; Puzey, Joshua R; Kim, Rebecca S et al. (2018) Selective trade-offs maintain alleles underpinning complex trait variation in plants. Science 361:475-478
Nelson, Thomas C; Monnahan, Patrick J; McIntosh, Mariah K et al. (2018) Extreme copy number variation at a tRNA ligase gene affecting phenology and fitness in yellow monkeyflowers. Mol Ecol :
Colicchio, J (2017) Transgenerational effects alter plant defence and resistance in nature. J Evol Biol 30:664-680
Monnahan, Patrick J; Kelly, John K (2017) The Genomic Architecture of Flowering Time Varies Across Space and Time in Mimulus guttatus. Genetics 206:1621-1635
Puzey, Joshua R; Willis, John H; Kelly, John K (2017) Population structure and local selection yield high genomic variation in Mimulus guttatus. Mol Ecol 26:519-535
Koseva, Boryana; Crawford, Daniel J; Brown, Keely E et al. (2017) The genetic breakdown of sporophytic self-incompatibility in Tolpis coronopifolia (Asteraceae). New Phytol 216:1256-1267
Case, Andrea L; Finseth, Findley R; Barr, Camille M et al. (2016) Selfish evolution of cytonuclear hybrid incompatibility in Mimulus. Proc Biol Sci 283:
Lee, Young Wha; Fishman, Lila; Kelly, John K et al. (2016) A Segregating Inversion Generates Fitness Variation in Yellow Monkeyflower (Mimulus guttatus). Genetics 202:1473-84

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