Multiple key components of the innate immune system are conserved across eukaryotes. In plants, the innate immune system serves as a barrier to inhibit both pathogen entry and multiplication. Despite the importance of the innate immune system, scientists still have a limited understanding of how plant immune complexes are assembled and regulated in response to pathogen perception. A key regulator of the plant immune system is the Arabidopsis gene RIN4. RIN4 is conserved among all land plants and acts to regulate immune perception of the bacterial pathogen Pseudomonas syringae pv. tomato in Arabidopsis. Preliminary data within this application demonstrate the purification of RIN4 protein complexes in the absence and presence of pathogen stimulus. Fifteen novel proteins were identified by mass spectrometry and multiple proteins were subsequently shown to interact with RIN4 by yeast two-hybrid. Arabidopsis knockout or overexpression lines for three of these RIN4 associated proteins display altered defense responses to P. syringae pv. tomato, suggesting that they are important components of the plant immune response. One RIN4 associated protein is the H+ATPase AHA1. Experiments indicate that RIN4 can directly regulate AHA1 enzymatic activity. RIN4 can work in concert with AHA1 to regulate leaf stomatal opening during the innate immune responses, thus blocking the entry of bacterial pathogens into the leaf interior. The central hypothesis of the proposed research is that RIN4 complex constituents will be key components controlling innate immune signaling. Several proposed experiments seek to understand RIN4 protein complex assembly and RIN4-mediated cellular signaling cascades using the P. syringae-Arabidopsis pathosystem. This pathosystem is an excellent model system to study eukaryotic innate immune signaling because of the extensive genetic resources available, the fast generation time of Arabidopsis, and the similarities between innate immune systems in plants and other eukaryotes.
The specific aims of this research application are the following: 1) Elucidate the mechanism RIN4 uses to regulate plasma membrane H+ATPase activity;2) Investigate the spatial and temporal components of the RIN4 protein network;3) Functionally characterize Arabidopsis RIN4 associated proteins.

Public Health Relevance

RIN4 is a central player in the regulation and activation of membrane-localized plant innate immune protein complexes. Multiple RIN4 associated proteins are widely conserved among eukaryotes and several have been implicated in immune signaling in mammals. Because there are significant similarities between innate immune systems in plants and other eukaryotes, we anticipate our findings will be broadly relevant to NIH's mission.

Agency
National Institute of Health (NIH)
Institute
National Institute of General Medical Sciences (NIGMS)
Type
Research Project (R01)
Project #
1R01GM092772-01
Application #
7861666
Study Section
Host Interactions with Bacterial Pathogens Study Section (HIBP)
Program Officer
Dunsmore, Sarah
Project Start
2010-05-01
Project End
2015-03-31
Budget Start
2010-05-01
Budget End
2011-03-31
Support Year
1
Fiscal Year
2010
Total Cost
$298,350
Indirect Cost
Name
University of California Davis
Department
Other Basic Sciences
Type
Schools of Earth Sciences/Natur
DUNS #
047120084
City
Davis
State
CA
Country
United States
Zip Code
95618
Zhang, Meixiang; Chiang, Yi-Hsuan; Toruño, Tania Y et al. (2018) The MAP4 Kinase SIK1 Ensures Robust Extracellular ROS Burst and Antibacterial Immunity in Plants. Cell Host Microbe 24:379-391.e5
Leibman-Markus, Meirav; Pizarro, Lorena; Schuster, Silvia et al. (2018) The intracellular nucleotide-binding leucine-rich repeat receptor (SlNRC4a) enhances immune signalling elicited by extracellular perception. Plant Cell Environ 41:2313-2327
Kadota, Yasuhiro; Liebrand, Thomas W H; Goto, Yukihisa et al. (2018) Quantitative phosphoproteomic analysis reveals common regulatory mechanisms between effector- and PAMP-triggered immunity in plants. New Phytol :
Henry, Elizabeth; Toruño, Tania Y; Jauneau, Alain et al. (2017) Direct and Indirect Visualization of Bacterial Effector Delivery into Diverse Plant Cell Types during Infection. Plant Cell 29:1555-1570
Yadeta, Koste A; Elmore, James M; Creer, Athena Y et al. (2017) A Cysteine-Rich Protein Kinase Associates with a Membrane Immune Complex and the Cysteine Residues Are Required for Cell Death. Plant Physiol 173:771-787
Zhang, Meixiang; Coaker, Gitta (2017) Harnessing Effector-Triggered Immunity for Durable Disease Resistance. Phytopathology 107:912-919
Luo, Xuming; Xu, Ning; Huang, Junkai et al. (2017) A Lectin Receptor-Like Kinase Mediates Pattern-Triggered Salicylic Acid Signaling. Plant Physiol 174:2501-2514
Michelmore, Richard; Coaker, Gitta; Bart, Rebecca et al. (2017) Foundational and Translational Research Opportunities to Improve Plant Health. Mol Plant Microbe Interact 30:515-516
Toruño, Tania Y; Stergiopoulos, Ioannis; Coaker, Gitta (2016) Plant-Pathogen Effectors: Cellular Probes Interfering with Plant Defenses in Spatial and Temporal Manners. Annu Rev Phytopathol 54:419-41
Niu, Dongdong; Lii, Yifan E; Chellappan, Padmanabhan et al. (2016) miRNA863-3p sequentially targets negative immune regulator ARLPKs and positive regulator SERRATE upon bacterial infection. Nat Commun 7:11324

Showing the most recent 10 out of 29 publications