Plants and animals rely on innate immunity to prevent potential infections by detection of pathogen-associated molecular patterns (PAMPs) through pattern recognition receptors (PRRs). In plants, PAMPs are perceived by cell-surface PRRs and mount PAMP- triggered immunity (PTI). Successful pathogens are able to suppress PTI by producing virulence effectors, which, in turn, are recognized by plant intracellular PRRs to initiate effector-triggered immunity (ETI). This complex host-microbe interaction is intricately intertwined with a wide range of environmental factors. For example, we found that the ambient temperature fluctuation regulates PTI and ETI in a distinct manner. We have developed an Arabidopsis cell system to express individual pathogen effectors for ETI or treat cells with purified PAMPs for PTI. In combination with enriched genetic and genomic resources in Arabidopsis, this synchronized and autonomous plant cell-single pathogen signal system holds significant promise for dissecting the complex regulation of signaling events in ETI and PTI. Our preliminary data strongly suggest the existence of differential early signaling mechanisms underlying two branches of plant innate immunity. In particular, we found that the specific members of a large gene family encoding calcium-dependent protein kinases (CDPKs) play pivotal roles in transducing calcium signature in ETI. Our proposed research will employ a multifaceted approach to further understand the signaling mechanisms underlying plant ETI and PTI.
The Specific Aims for this application are the following: 1) Decipher the distinct and convergent gene regulation in ETI and PTI;2) Elucidate the functions of CDPKs in ETI signaling;3) Dissect the ambient temperature regulation of innate immunity. Plant PTI and ETI are most similar to """"""""Toll-like receptor"""""""" and """"""""NOD-like receptor"""""""" innate immune signaling pathways in mammals respectively. Thus, our research will contribute to a general understanding of innate immunity and improve our ability to prevent and control infectious diseases.

Public Health Relevance

Understanding the intricately intertwined signal transduction networks acting downstream of various host immune sensors could provide novel avenues to prevent and control infectious diseases. Given that plant cell-surface and intracellular immune sensors are most similar to Toll-like receptor and NOD-like receptor in mammals respectively, our research will have broad impact on the understanding of signaling mechanisms of animal innate immunity.

Agency
National Institute of Health (NIH)
Institute
National Institute of General Medical Sciences (NIGMS)
Type
Research Project (R01)
Project #
5R01GM092893-05
Application #
8607963
Study Section
Molecular and Integrative Signal Transduction Study Section (MIST)
Program Officer
Dunsmore, Sarah
Project Start
2010-04-01
Project End
2015-01-31
Budget Start
2014-02-01
Budget End
2015-01-31
Support Year
5
Fiscal Year
2014
Total Cost
$226,501
Indirect Cost
$70,130
Name
Texas A&M University
Department
Biochemistry
Type
Schools of Earth Sciences/Natur
DUNS #
078592789
City
College Station
State
TX
Country
United States
Zip Code
77845
Jamieson, Pierce A; Shan, Libo; He, Ping (2018) Plant cell surface molecular cypher: Receptor-like proteins and their roles in immunity and development. Plant Sci 274:242-251
Zhou, Jinggeng; Liu, Derui; Wang, Ping et al. (2018) Regulation of Arabidopsis brassinosteroid receptor BRI1 endocytosis and degradation by plant U-box PUB12/PUB13-mediated ubiquitination. Proc Natl Acad Sci U S A 115:E1906-E1915
Mang, Hyunggon; Feng, Baomin; Hu, Zhangjian et al. (2017) Differential Regulation of Two-Tiered Plant Immunity and Sexual Reproduction by ANXUR Receptor-Like Kinases. Plant Cell 29:3140-3156
Feng, Baomin; Ma, Shisong; Chen, Sixue et al. (2016) PARylation of the forkhead-associated domain protein DAWDLE regulates plant immunity. EMBO Rep 17:1799-1813
Li, Bo; Meng, Xiangzong; Shan, Libo et al. (2016) Transcriptional Regulation of Pattern-Triggered Immunity in Plants. Cell Host Microbe 19:641-50
Meng, Xiangzong; Zhou, Jinggeng; Tang, Jiao et al. (2016) Ligand-Induced Receptor-like Kinase Complex Regulates Floral Organ Abscission in Arabidopsis. Cell Rep 14:1330-1338
de Oliveira, Marcos V V; Xu, Guangyuan; Li, Bo et al. (2016) Specific control of Arabidopsis BAK1/SERK4-regulated cell death by protein glycosylation. Nat Plants 2:15218
Feng, Baomin; Liu, Chenglong; Shan, Libo et al. (2016) Protein ADP-Ribosylation Takes Control in Plant-Bacterium Interactions. PLoS Pathog 12:e1005941
Ma, Xiyu; Xu, Guangyuan; He, Ping et al. (2016) SERKing Coreceptors for Receptors. Trends Plant Sci 21:1017-1033
Li, Bo; Jiang, Shan; Yu, Xiao et al. (2015) Phosphorylation of trihelix transcriptional repressor ASR3 by MAP KINASE4 negatively regulates Arabidopsis immunity. Plant Cell 27:839-56

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