The long term goal of this proposal is the development of tools enabling high throughput single-molecule fluorescence spectroscopy.
The specific aims of this proposal are: (1) Develop an optical setup for 3 color, 48 spot excitation and detection. (2) Develop a 48 pixel custom single-photon avalanche photodiode array (48p-SPADA). (3) Develop field-programmable-gate-array (FPGA) data reduction algorithms for up to 144 channels single-molecule burst analysis. (4) Develop high-throughput fluorescence (cross)-correlation spectroscopy data analysis algorithms in graphical processing unit (GPU). (5) Study fast and transient events in transcription initiation and elongation, focusing on the conformation and interactions of 70 with the bacterial RNAP holoenzyme. The expected outcome of this proposal is an increase in throughput for single molecule fluorescence techniques of a factor 48. This will include most single-molecule fluorescence techniques such as single-molecule fluorescence resonant energy transfer (smFRET) and microsecond alternating laser excitation (s-ALEX) as well as fluorescence correlation spectroscopy (FCS). The impact of these developments will be twofold. First, single-molecule fluorescence spectroscopy methods will become attractive to a larger research community, which will be able to use is as an efficient discovery and analytical tool. Additionally, it will give access to new temporal regimes that are unattainable with the current technology. Second, the leap in throughput (by a factor 48) will render this technology attractive for point-of-care diagnostics and large scale screening assays used by the biotechnology and pharmaceutical industry. For these reasons, this proposal is very likely to significantly impact the progresses of many areas of biological research including drug discovery and development as well as diagnostics, thus helping increase our understanding of life processes and lays the foundation for advances in disease diagnosis, treatment, and prevention.

Public Health Relevance

The development of new detectors for high-throughput single-fluorescence molecule spectroscopy will result in accelerated measurements and enable highly parallel studies. This will allow mainstream researchers to take advantage of this powerful technology, thus accelerating the pace of discovery. It will also make high-throughput diagnostics and drug screening applications of single-molecule a practical reality, therefore permitting advances in disease diagnosis, treatment, and prevention.

Agency
National Institute of Health (NIH)
Institute
National Institute of General Medical Sciences (NIGMS)
Type
Research Project (R01)
Project #
5R01GM095904-04
Application #
8814242
Study Section
Enabling Bioanalytical and Imaging Technologies Study Section (EBIT)
Program Officer
Deatherage, James F
Project Start
2012-05-01
Project End
2017-02-28
Budget Start
2015-03-01
Budget End
2016-02-29
Support Year
4
Fiscal Year
2015
Total Cost
$406,856
Indirect Cost
$99,349
Name
University of California Los Angeles
Department
Chemistry
Type
Schools of Arts and Sciences
DUNS #
092530369
City
Los Angeles
State
CA
Country
United States
Zip Code
90095
Ingargiola, Antonino; Segal, Maya; Gulinatti, Angelo et al. (2018) 48-spot single-molecule FRET setup with periodic acceptor excitation. J Chem Phys 148:123304
Ceccarelli, Francesco; Gulinatti, Angelo; Labanca, Ivan et al. (2018) Red-Enhanced Photon Detection Module Featuring a 32 × 1 Single-Photon Avalanche Diode Array. IEEE Photonics Technol Lett 30:557-560
Lerner, Eitan; Cordes, Thorben; Ingargiola, Antonino et al. (2018) Toward dynamic structural biology: Two decades of single-molecule Förster resonance energy transfer. Science 359:
Lerner, Eitan; Ingargiola, Antonino; Weiss, Shimon (2018) Characterizing highly dynamic conformational states: The transcription bubble in RNAP-promoter open complex as an example. J Chem Phys 148:123315
Ingargiola, Antonino; Peronio, Pietro; Lerner, Eitan et al. (2017) 16-Ch Time-resolved Single-Molecule Spectroscopy Using Line Excitation. Proc SPIE Int Soc Opt Eng 10071:
Ceccarelli, Francesco; Gulinatti, Angelo; Labanca, Ivan et al. (2017) Development and characterization of an 8×8 SPAD-array module for gigacount per second applications. Proc SPIE Int Soc Opt Eng 10229:
Ingargiola, Antonino; Lerner, Eitan; Chung, SangYoon et al. (2017) Multispot single-molecule FRET: High-throughput analysis of freely diffusing molecules. PLoS One 12:e0175766
Lerner, Eitan; Ingargiola, Antonino; Lee, Jookyung J et al. (2017) Different types of pausing modes during transcription initiation. Transcription 8:242-253
Ceccarelli, Francesco; Gulinatti, Angelo; Labanca, Ivan et al. (2016) Gigacount/second Photon Detection Module Based on an 8 × 8 Single-Photon Avalanche Diode Array. IEEE Photonics Technol Lett 28:1002-1005
Ingargiola, Antonino; Lerner, Eitan; Chung, SangYoon et al. (2016) FRETBursts: An Open Source Toolkit for Analysis of Freely-Diffusing Single-Molecule FRET. PLoS One 11:e0160716

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