Critical physiological and pathological processes, such as wound healing, blood vessel formation and cancer metastasis, rely on directed collective cell migrations, whereby groups of cells collectively polarize and move together in an orderly fashion. The ability of cell collectives to migrate directionally is determined in part by the tissue-specific transcriptional inputs that define the complement of expressed genes and thus their competence to migrate. The long-term goal of this project is to understand how tissue-specific transcription regulators control the basic cellular processes underlying directed collective cell migration. To this aim, the simplified embryos of a chordate species, the ascidian Ciona intestinalis, will be used to study the migration of pre- cardiac mesoderm cells, called ?trunk ventral cells? (TVCs). The TVCs provide the simplest possible model of directed collective cell migration in live embryos. On each side of the embryo, only two cells migrate together and display a clear Leader-Trailer polarity aligned with the direction of migration: the leader TVC displays a broad leading edge and more conspicuous protrusions than the trailer. It was previously established that Mesp, Fibroblast growth factor (Fgf) signaling and FoxF transcriptional inputs determine the ability of TVCs to migrate. Moreover, TVCs migrate strictly between the endodermal and ectodermal germ layers, a hallmark of mesodermal cardiac progenitors. It was determine that these surrounding tissues contribute to canalizing TVCs' innate motility towards collective polarity and directed migration. The goal of the proposed research is to understand how transcriptionally-controlled intrinsic TVC properties interface with extrinsic signals to determine collective polarity and directed migration in the embryo. Preliminary studies suggested that the gene encoding the discoidin domain receptor (Ddr) is upregulated by Mesp, FGF and FoxF transcriptional inputs in the TVCs, where it promotes adhesion to the epidermis. Using newly developed quantitative imaging methods, the detailed mechanisms controlling Ddr expression, localization and activity will be analyzed. The hypothesis that a cell-autonomous antagonism between Ddr and vascular endothelial growth factor receptor (Vegfr) signaling positions the migrating TVCs between the epidermis and endoderm will be tested. Preliminary observations suggest that Ddr promotes adhesion to the epidermis by regulating vesicle trafficking. The hypothesis that Ddr acts in Rab4/Rab11-positive endosomes to promote the recycling of integrins to the plasma membrane will be tested. Finally, the functions of regulated candidate effectors of collective migration will be studied extensively using TVC-specific CRISPR/Cas9-mediated loss-of-function assays and high-content phenotypic analyses. A provisional model of the biomolecular network controlling the subcellular processes underlying TVC behavior will be built. Particular attention will be paid to the candidate modulators of Ddr, Vegfr and integrin functions. Completion of this project will illuminate the systems' level mechanisms linking intrinsic transcriptional inputs and extrinsic signals to define cell-specific behaviors.

Public Health Relevance

In animals, diverse morphogenetic processes referred to as collective cell migrations are essential for physiological and pathological phenomena such as embryonic development, blood vessel formation, wound healing and cancer metastasis. We propose to study the mechanisms that control directed collective cell migration using the simplest possible model, the polarized pairs of migrating cardiac progenitors in embryos of the tunicate Ciona intestinalis, an invertebrate chordate closely related to vertebrates. Studies using this simple model will decipher the basic mechanisms driving collective cell migration, with potential applications for regenerative medicine and the prevention of cancer metastasis.

Agency
National Institute of Health (NIH)
Institute
National Institute of General Medical Sciences (NIGMS)
Type
Research Project (R01)
Project #
2R01GM096032-06A1
Application #
9176375
Study Section
Development - 1 Study Section (DEV1)
Program Officer
Hoodbhoy, Tanya
Project Start
2010-09-23
Project End
2020-07-31
Budget Start
2016-09-02
Budget End
2017-07-31
Support Year
6
Fiscal Year
2016
Total Cost
$317,215
Indirect Cost
$114,512
Name
New York University
Department
Biology
Type
Schools of Arts and Sciences
DUNS #
041968306
City
New York
State
NY
Country
United States
Zip Code
10012
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Gandhi, Shashank; Razy-Krajka, Florian; Christiaen, Lionel et al. (2018) CRISPR Knockouts in Ciona Embryos. Adv Exp Med Biol 1029:141-152
Gandhi, Shashank; Haeussler, Maximilian; Razy-Krajka, Florian et al. (2017) Evaluation and rational design of guide RNAs for efficient CRISPR/Cas9-mediated mutagenesis in Ciona. Dev Biol 425:8-20
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Stolfi, Alberto; Sasakura, Yasunori; Chalopin, Domitille et al. (2015) Guidelines for the nomenclature of genetic elements in tunicate genomes. Genesis 53:1-14
Gline, Stephanie; Kaplan, Nicole; Bernadskaya, Yelena et al. (2015) Surrounding tissues canalize motile cardiopharyngeal progenitors towards collective polarity and directed migration. Development 142:544-54
Stolfi, Alberto; Ryan, Kerrianne; Meinertzhagen, Ian A et al. (2015) Migratory neuronal progenitors arise from the neural plate borders in tunicates. Nature 527:371-4
Diogo, Rui; Kelly, Robert G; Christiaen, Lionel et al. (2015) A new heart for a new head in vertebrate cardiopharyngeal evolution. Nature 520:466-73

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