Chronic inflammation is a major cause of cancer, diabetes, and neurodegeneration. Inflammation is a complex topic, with both positive and negative effects on disease and therapy, and if we are to understand the pathological results and therapeutic benefits we need to better understand the mechanisms of where inflammation originates. The big picture in which this proposal is set is to understand the mechanisms of inflammation as they relate to regulated cell death. This application seeks to substantially advance current approaches, concepts, and technology to delve deeply into the inflammatory consequences of cell death and the connections between the cell death mechanisms. We combine biochemistry, cell biology, genome editing, non-animal models of inflammation, protein engineering, and chemical biology to explore linked pro- and anti- inflammatory cell death mechanisms. We propose that a robust and quantitative approach to defining inflammation is an important innovative quality of this proposal. The main goals of this proposal are to 1) compute the difference in inflammatory mediators released as a result of apoptosis, pyroptosis and necroptosis in macrophages, 2) define the mechanism of cytokine release from cells undergoing inflammatory cell death, and 3) understand the activation mechanisms of caspases in pyroptosis, and synthesize highly selective fluorescent probes to allow for direct visualization of specific caspase activation.

Public Health Relevance

Inflammation has both positive and negative functions in disease and disease therapy. This project seeks to explore fundamental links between regulated cell death mechanisms and the origins of inflammation.

Agency
National Institute of Health (NIH)
Institute
National Institute of General Medical Sciences (NIGMS)
Type
Research Project (R01)
Project #
5R01GM099040-07
Application #
9752563
Study Section
Cellular Signaling and Regulatory Systems Study Section (CSRS)
Program Officer
Maas, Stefan
Project Start
2012-05-01
Project End
2021-07-31
Budget Start
2019-08-01
Budget End
2020-07-31
Support Year
7
Fiscal Year
2019
Total Cost
Indirect Cost
Name
Sanford Burnham Prebys Medical Discovery Institute
Department
Type
DUNS #
020520466
City
La Jolla
State
CA
Country
United States
Zip Code
92037
Ramirez, Monica L Gonzalez; Poreba, Marcin; Snipas, Scott J et al. (2018) Extensive peptide and natural protein substrate screens reveal that mouse caspase-11 has much narrower substrate specificity than caspase-1. J Biol Chem 293:7058-7067
Kasperkiewicz, Paulina; Ko?t, Sonia; Janiszewski, Tomasz et al. (2018) Determination of extended substrate specificity of the MALT1 as a strategy for the design of potent substrates and activity-based probes. Sci Rep 8:15998
Ramirez, Monica L Gonzalez; Salvesen, Guy S (2018) A primer on caspase mechanisms. Semin Cell Dev Biol 82:79-85
Poreba, Marcin; Groborz, Katarzyna; Navarro, Mario et al. (2018) Caspase selective reagents for diagnosing apoptotic mechanisms. Cell Death Differ :
Kasperkiewicz, Paulina; Altman, Yoav; D'Angelo, Maximiliano et al. (2017) Toolbox of Fluorescent Probes for Parallel Imaging Reveals Uneven Location of Serine Proteases in Neutrophils. J Am Chem Soc 139:10115-10125
Lee, Peishan; Zhu, Zilu; Hachmann, Janna et al. (2017) Differing Requirements for MALT1 Function in Peripheral B Cell Survival and Differentiation. J Immunol 198:1066-1080
Hachmann, Janna; Salvesen, Guy S (2016) The Paracaspase MALT1. Biochimie 122:324-38
Poreba, Marcin; Solberg, Rigmor; Rut, Wioletta et al. (2016) Counter Selection Substrate Library Strategy for Developing Specific Protease Substrates and Probes. Cell Chem Biol 23:1023-35
Brumatti, Gabriela; Ma, Chunyan; Lalaoui, Najoua et al. (2016) The caspase-8 inhibitor emricasan combines with the SMAC mimetic birinapant to induce necroptosis and treat acute myeloid leukemia. Sci Transl Med 8:339ra69
Salvesen, Guy S; Hempel, Anne; Coll, Nuria S (2016) Protease signaling in animal and plant-regulated cell death. FEBS J 283:2577-98

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