Role of PTEN in Mitosis and Chromosome Stability Project Summary The chromatin environment shapes DNA-based processes and recent studies reveal that the PTEN tumor suppressor con- trols both DNA duplication/segregation and chromatin remodeling. A fundamental question is how PTEN mediates the interplay between the genome and the epigenome to ensure faithful transmission of genetic materials. Our long-term goal is to illuminate the function of PTEN in maintaining mitotic chromosome stability and to understand why this function is a major driving force in tumor suppression. The overall objective of this project is to identify critical regulatory elements in both mitosis-intrinsic machinery and global chromatin architecture that orchestrate under the control of PTEN to pro- mote mitotic fidelity. In support of this goal, our recent studies show that depletion of PTEN results in spindle shortening and pole fragmentation, accompanied by misalignment and non-disjunction of chromosomes, leading to catastrophic mi- totic failure or polyploidy. These observations suggest that PTEN is directly involved in mediating the interaction between chromosomes and the mitotic spindle. In addition, metaphase cells lacking PTEN exhibit prominent chromosome entan- glement, acentric anaphase bridges, centromere breakage and premature chromatid separation, indicating structural chromosome instability likely due to impaired chromatin architecture. Interestingly, our published data demonstrate that PTEN regulates histone anchorage and modification on chromatin and thereby affects the chromatin compaction status. These results collectively support our hypothesis that PTEN maintains structural and numerical chromosome stability by coordinating mitotic chromatin compaction and mitotic spindle assembly for proper chromosome alignment and segrega- tion. We will test this hypothesis by pursuing two specific aims. In the first aim, we will demonstrate impairment of mitotic chromatin compaction and epigenetic deregulation of kinetochore assembly as a prevailing phenotypic conse- quence of PTEN deficiency. High-resolution imaging and biochemical approaches, in combination with two knock-in animal models developed in our laboratory, will be employed to analyze and dissect PTEN function in promoting mitotic chromatin condensation. In the second aim, we will delineate the mechanism underlying the functional interaction be- tween interrelated PTEN pathways in governing spindle activity and chromosome behavior. Using a comprehensive set of cellular and molecular biology approaches, coupled with newly generated transgenic mouse strains, we will depict a PTEN-associated mitotic signaling network comprised of both structural and functional regulatory elements that act in concert to guard the genome. Successful completion of these aims will fundamentally advance our mechanistic under- standing of the multifaceted function of PTEN in mitosis and chromosome stability. Data from our studies will illustrate how PTEN deficiency triggers a deteriorative interaction between deregulated mitotic machinery and unfavorable chroma- tin milieus, leading to structural and numerical chromosome aberrations. Newly discovered mitotic pathways and targets in this innovative project will contribute to precision medicine through tailoring targeted therapies based on the genetic and epigenetic signatures of each cancer patient.
Role of PTEN in Mitosis and Chromosome Stability Project Narrative Cancer evolves from detrimental genetic and epigenetic alterations. This study will reveal a cancer evolution mechanism of aberrant chromatin-chromosome interplay by focusing on the central process of genetic transmission. Our findings will provide innovative implications for personalized treatment of cancer patients tailored to the genetic and epigenetic signa- ture of each tumor.
| Brandmaier, Andrew; Hou, Sheng-Qi; Demaria, Sandra et al. (2017) PTEN at the interface of immune tolerance and tumor suppression. Front Biol (Beijing) 12:163-174 |
| Hou, Sheng-Qi; Ouyang, Meng; Brandmaier, Andrew et al. (2017) PTEN in the maintenance of genome integrity: From DNA replication to chromosome segregation. Bioessays 39: |
| Brandmaier, Andrew; Hou, Sheng-Qi; Shen, Wen H (2017) Cell Cycle Control by PTEN. J Mol Biol 429:2265-2277 |
| Zhang, Zhong; Hou, Sheng-Qi; He, Jinxue et al. (2016) PTEN regulates PLK1 and controls chromosomal stability during cell division. Cell Cycle 15:2476-85 |
| Yu, Sonia; Yang, Fan; Shen, Wen H (2016) Genome maintenance in the context of 4D chromatin condensation. Cell Mol Life Sci 73:3137-50 |
| He, Jinxue; Zhang, Zhong; Ouyang, Meng et al. (2016) PTEN regulates EG5 to control spindle architecture and chromosome congression during mitosis. Nat Commun 7:12355 |
| Kang, Xi; Song, Chang; Du, Xiao et al. (2015) PTEN stabilizes TOP2A and regulates the DNA decatenation. Sci Rep 5:17873 |
| He, Jinxue; Kang, Xi; Yin, Yuxin et al. (2015) PTEN regulates DNA replication progression and stalled fork recovery. Nat Commun 6:7620 |
| Chen, Zhu Hong; Zhu, Minglu; Yang, Jingyi et al. (2014) PTEN interacts with histone H1 and controls chromatin condensation. Cell Rep 8:2003-2014 |
| Sun, Zhuo; Huang, Chuanxin; He, Jinxue et al. (2014) PTEN C-terminal deletion causes genomic instability and tumor development. Cell Rep 6:844-54 |
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