The human infant is typically sterile in utero and then born into a world dominated by microbes, which selectively assume residence of the body surfaces and enteric tract of the newborn. The assembly of the early human microbiome is particularly an issue relevant to the very low birth weight (VLBW;<1500 g) infant, where infection and activation of immunological and inflammatory responses produce acute morbidity and mor- tality and exacerbate co-morbid diseases of the eyes, enteric tract, and central nervous system. However, the selective forces that alter primary colonization and secondary persistence of more or less pathogenic microbes within the developing microbiota are not well understood, particularly in humans. Specifically, the extent to which colonization is recognized by the adaptive immune response and how the adaptive responses contribute to shaping the microbiome of the premature infant, and how the microbiome influences concurrent and subse- quent adaptive immune development, is poorly understood. The immediate goal of the proposed research is to identify antigens from the pioneering microbiome of the intestine in the preterm infant that are recognized by natural birth antibodies and the adaptive humoral response. This work will then determine whether and how the peripheral and mucosal antibody response alters the dynamics of secondary colonization. The central hypoth- esis is that natural antibodies present at birth modify primary colonization while antigens of pioneering Gram negative enteric bacteria drive adaptive antibody responses that alter secondary colonization and long-term immune memory. The hypothesis will be addressed through an innovative combination of molecular tools in the following aims: 1) To determine the relationship between natural and adaptive antibodies and colonization of the VLBW infant intestinal tract, 2) To determine the specificity of breast milk IgA to the breast milk microbi- ome and the early intestinal microbiome in the VLBW infant, and 3) To measure the specific antigens of the neonatal microbiome recognized by antibodies in the first year of life. These studies will uniquely define the constellation of antigens recognized by preterm infant and maternal natural and adaptive antibodies as the in- fant becomes colonized. By determining the dynamic interface between mucosal antibody response and spe- cific antigens recognized by the newborn human host, new interventions may be envisioned through which to alter primary and secondary colonization of infants through stimulation of natural and adaptive antibodies. In turn these advents may lead to approaches to alter long-term outcomes including infectious diseases, allergy, and metabolic syndrome.
Recognition of colonizing microbes by the immune system following birth will have lasting consequences for the risk of infection, allergy, and metabolism. Premature infants are particularly at risk of these health problems, in the short term, in the form of bronchopulmonary dysplasia, necrotizing enterocolitis, and sepsis, and in the longer term, disorders of allergy, metabolism, and susceptibility to infectious diseases. Understanding immune recognition of microbial colonization may provide new insights into how to improve immunity and decrease future health problems.
|Younge, Noelle E; Araújo-Pérez, Félix; Brandon, Debra et al. (2018) Early-life skin microbiota in hospitalized preterm and full-term infants. Microbiome 6:98|
|Zaengle-Barone, Jacqueline M; Jackson, Abigail C; Besse, David M et al. (2018) Copper Influences the Antibacterial Outcomes of a ?-Lactamase-Activated Prochelator against Drug-Resistant Bacteria. ACS Infect Dis 4:1019-1029|
|Kelly, Matthew S; Surette, Michael G; Smieja, Marek et al. (2018) Pneumococcal Colonization and the Nasopharyngeal Microbiota of Children in Botswana. Pediatr Infect Dis J 37:1176-1183|
|Younge, Noelle; Yang, Qing; Seed, Patrick C (2017) Enteral High Fat-Polyunsaturated Fatty Acid Blend Alters the Pathogen Composition of the Intestinal Microbiome in Premature Infants with an Enterostomy. J Pediatr 181:93-101.e6|
|McCann, Jessica R; Mason, Stanley N; Auten, Richard L et al. (2016) Early-Life Intranasal Colonization with Nontypeable Haemophilus influenzae Exacerbates Juvenile Airway Disease in Mice. Infect Immun 84:2022-2030|
|Cotten, Charles M (2016) Adverse consequences of neonatal antibiotic exposure. Curr Opin Pediatr 28:141-9|
|Seed, Patrick C (2016) Do Bacteria in the Gut Set the Stage for Who Gets Viral Bronchiolitis and Its Severity? Pediatrics 138:|
|Arshad, Mehreen; Goller, Carlos C; Pilla, Danielle et al. (2016) Threading the Needle: Small-Molecule Targeting of a Xenobiotic Receptor to Ablate Escherichia coli Polysaccharide Capsule Expression Without Altering Antibiotic Resistance. J Infect Dis 213:1330-9|
|Hartz, Lacey E; Bradshaw, Wanda; Brandon, Debra H (2015) Potential NICU Environmental Influences on the Neonate's Microbiome: A Systematic Review. Adv Neonatal Care 15:324-35|
|Pineda, Leslie C; Hornik, Christoph P; Seed, Patrick C et al. (2015) Association between positive urine cultures and necrotizing enterocolitis in a large cohort of hospitalized infants. Early Hum Dev 91:583-6|
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