The centromere is a unique chromatin domain defined by the incorporation of a centromere specific nucleosome containing centromere protein-A (CENP-A). The centromere recruits the mitotic kinetochore to ensure the equal segregation of chromosomes during mitosis. The location of the centromere along the chromosome is determined by an epigenetic mechanism that relies on the CENP-A nucleosome. New CENP-A nucleosomes are assembled into the centromeres of dividing cells during a discreet time in early G1. Propagation of the centromere requires assembly of new CENP-A nucleosomes prior to DNA replication to avoid the loss of CENP-A nucleosomes through their successive dilution. Previously we determined Mis18 complex recruitment is the defining step in epigenetic inheritance. This proposal will explore the mechanism by which new CENP-A is recruited to centromeres through the recruitment of the Mis18 complex. Canonical histones are subject to multiple posttranslational modifications (PTMs) that drive the recruitment of chromatin associated factors and modify the function of the underlying chromatin. We will explore the function of two newly identified PTMs of the CENP-A tail, amino-terminal trimethylation and dual phosphorylation of serine 16 and 18. The experiments proposed here will significantly advance our understanding of the epigenetic mechanism of centromere inheritance. Furthermore, the impact of this work will extend beyond the centromere to provide significant insight into the propagation of epigenetic information encoded by acetylation and methylation or other histone variants.

Public Health Relevance

Genomic instability is a major factor in the genesis and progression of cancer and can result from the missegregation of chromosomes during mitosis. Chromosome segregation is controlled by the centromere, a unique domain on the chromosomes, the core element of which is a unique nucleosome containing centromere protein-A (CENP- A). The experiments in this proposal will examine the earliest steps in establishing the centromere. They will identify a novel pathway for the stable inheritance of CENP-A nucleosomes with the potential to have important implications for the inheritance of a variety of epigenetic marks. In addition these experiments will identify the function of novel posttranslational modifications involved in the centromere function. The long-term goal is to understand how the perturbation of the centromere can lead to chromosome instability and cancer.

Agency
National Institute of Health (NIH)
Institute
National Institute of General Medical Sciences (NIGMS)
Type
Research Project (R01)
Project #
1R01GM111907-01
Application #
8765120
Study Section
Nuclear and Cytoplasmic Structure/Function and Dynamics Study Section (NCSD)
Program Officer
Carter, Anthony D
Project Start
2014-08-01
Project End
2019-07-31
Budget Start
2014-08-01
Budget End
2015-07-31
Support Year
1
Fiscal Year
2014
Total Cost
$299,600
Indirect Cost
$107,100
Name
University of Virginia
Department
Biochemistry
Type
Schools of Medicine
DUNS #
065391526
City
Charlottesville
State
VA
Country
United States
Zip Code
22904
Srivastava, Shashank; Zasadzi?ska, Ewelina; Foltz, Daniel R (2018) Posttranslational mechanisms controlling centromere function and assembly. Curr Opin Cell Biol 52:126-135
Zasadzi?ska, Ewelina; Huang, Jiehuan; Bailey, Aaron O et al. (2018) Inheritance of CENP-A Nucleosomes during DNA Replication Requires HJURP. Dev Cell 47:348-362.e7
Srivastava, Shashank; Foltz, Daniel R (2018) Posttranslational modifications of CENP-A: marks of distinction. Chromosoma 127:279-290
Zasadzi?ska, Ewelina; Foltz, Daniel R (2017) Orchestrating the Specific Assembly of Centromeric Nucleosomes. Prog Mol Subcell Biol 56:165-192
Sathyan, Kizhakke M; Fachinetti, Daniele; Foltz, Daniel R (2017) ?-amino trimethylation of CENP-A by NRMT is required for full recruitment of the centromere. Nat Commun 8:14678
Barnhart-Dailey, Meghan C; Trivedi, Prasad; Stukenberg, P Todd et al. (2017) HJURP interaction with the condensin II complex during G1 promotes CENP-A deposition. Mol Biol Cell 28:54-64
Shrestha, Roshan L; Ahn, Grace S; Staples, Mae I et al. (2017) Mislocalization of centromeric histone H3 variant CENP-A contributes to chromosomal instability (CIN) in human cells. Oncotarget 8:46781-46800
Zasadzi?ska, Ewelina; Foltz, Daniel R (2016) Centromeres of a Different CAL-ibre. Dev Cell 37:105-6
Nardi, Isaac K; Zasadzi?ska, Ewelina; Stellfox, Madison E et al. (2016) Licensing of Centromeric Chromatin Assembly through the Mis18?-Mis18? Heterotetramer. Mol Cell 61:774-787
Bailey, Aaron O; Panchenko, Tanya; Shabanowitz, Jeffrey et al. (2016) Identification of the Post-translational Modifications Present in Centromeric Chromatin. Mol Cell Proteomics 15:918-31

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