Studying mechanisms underlying asymmetric histone inheritance during asymmetric division of Drosophila male germ line stem cell Project Summary: A broad definition for """"""""epigenetic phenomenon"""""""" refers to effects on gene expression or function that are inheritable through cell divisions without altering the primary DNA sequences. Epigenetic mechanisms play important roles in regulating stem cell identity and activity. Failure in appropriate epigenetic regulation leads to abnormalities in stem cell behavior, which underlies many diseases such as diabetes, muscular dystrophy, neurodegenerative disease, infertility, and many forms of cancer. However, a long-standing question has been whether stem cells retain their epigenetic memory during divisions. And if so, could abolishment of stem cell- specific epigenetic memory lead to diseases? Many types of stem cells undergo asymmetric cell divisions to give rise to two daughter cells with distinct cell fates: a self-renewed stem cel and to another daughter cell that differentiates. Recently we found that during the asymmetric division of Drosophila male germ line stem cell (GSC), the preexisting histone 3 (H3) are selectively segregated to the GSC whereas newly synthesized H3 are enriched in the differentiating daughter cell. Our studies provide the first direct evidence that stem cells retain preexisting histones during asymmetric cell divisions in vivo, which may contribute to maintain their unique epigenetic memory. We hypothesize a two-step model for asymmetric H3 segregation: during S phase preexisting histones and newly synthesized histones are differentially incorporated to two sets of sister chromatids;then, during mitosis the set of siste chromatids incorporating preexisting histones are segregated to GSCs, while the other set of sister chromatids enriched with newly synthesized histones are partitioned to the daughter cell which undergoes differentiation. Here we propose to use molecular genetics, cell biology and biophysical approaches to understand mechanisms and biological significance underlying both steps, which will have far-reaching impact on understanding epigenetic inheritance in stem cell maintenance and differentiation.

Public Health Relevance

It is critical to understand how stem cells maintain their normal identity and activity through many cell divisions, because this knowledge will help understand how failure in doing so leads to various human diseases. Our proposed research is addressing how stem cells retain their unique properties by inheriting specific epigenetic information.

National Institute of Health (NIH)
National Institute of General Medical Sciences (NIGMS)
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Development - 1 Study Section (DEV1)
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Haynes, Susan R
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Johns Hopkins University
Schools of Arts and Sciences
United States
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Gleason, Ryan J; Anand, Amit; Kai, Toshie et al. (2018) Protecting and Diversifying the Germline. Genetics 208:435-471
Snedeker, Jonathan; Wooten, Matthew; Chen, Xin (2017) The Inherent Asymmetry of DNA Replication. Annu Rev Cell Dev Biol 33:291-318
Xie, Jing; Wooten, Matthew; Tran, Vuong et al. (2017) Breaking Symmetry - Asymmetric Histone Inheritance in Stem Cells. Trends Cell Biol 27:527-540
Kahney, Elizabeth W; Ranjan, Rajesh; Gleason, Ryan J et al. (2017) Symmetry from Asymmetry or Asymmetry from Symmetry? Cold Spring Harb Symp Quant Biol 82:305-318
Xiao, Jie; DufrĂȘne, Yves F (2016) Optical and force nanoscopy in microbiology. Nat Microbiol 1:16186
Xie, Jing; Wooten, Matthew; Tran, Vuong et al. (2015) Histone H3 Threonine Phosphorylation Regulates Asymmetric Histone Inheritance in the Drosophila Male Germline. Cell 163:920-33
Feng, Lijuan; Chen, Xin (2015) Epigenetic regulation of germ cells-remember or forget? Curr Opin Genet Dev 31:20-7