Skin melanocytes and other pigment-producing cells accomplish their main physiological task of melanin biosynthesis inside highly specialized compartments known as melanosomes. Melanosomes belong to a group of functionally specialized, cell-type-specific organelles that are derived from the endosomal-lysosomal system and known as lysosome-related organelles (LROs). Defective melanosome biogenesis underlies several forms of oculocutaneous albinism, with its typical manifestations in skin (reduced tanning ability, increased risk of developing skin cancer) and eyes (reduced visual acuity, nystagmus), often as part of syndromes - such as Hermansky-Pudlak syndrome (HPS) - that also include clinical manifestations caused by defective biogenesis of other LROs. The short-term goal of this project is to understand the functional specialization of selected components of the molecular machinery for protein trafficking in the endosomal-lysosomal system of most cell types, such that these components can also mediate the biogenesis of cell-type-specific LROs such as melanosomes. For example, genetic defects in some of the genes encoding ubiquitously expressed subunits of Adaptor Protein (AP)-3 and Biogenesis of Lysosome-related Organelles Complex (BLOC)-1 cause various forms of HPS. In the first Specific Aim, we will characterize some poorly understood molecular determinants for AP-3 and BLOC-1 function in melanosome biogenesis. In the second Specific Aim, we will characterize novel components of this molecular machinery. In the third Specific Aim, we will attempt to define the existence of novel LROs in other cell types.

Public Health Relevance

The proposed research is likely to improve our understanding of the molecular machinery that makes specialized intracellular compartments such as those where melanins are produced by melanocytes and other pigment-producing cells of our body. Defective melanosome biogenesis underlies several forms of human disease characterized by manifestations in skin, such as reduced tanning ability and propensity to develop skin cancer, as well as in the eyes, such as reduced visual acuity.

Agency
National Institute of Health (NIH)
Institute
National Institute of General Medical Sciences (NIGMS)
Type
Research Project (R01)
Project #
5R01GM112942-04
Application #
9380965
Study Section
Arthritis, Connective Tissue and Skin Study Section (ACTS)
Program Officer
Maas, Stefan
Project Start
2014-12-01
Project End
2019-11-30
Budget Start
2017-12-01
Budget End
2019-11-30
Support Year
4
Fiscal Year
2018
Total Cost
Indirect Cost
Name
University of California Los Angeles
Department
Genetics
Type
Schools of Medicine
DUNS #
092530369
City
Los Angeles
State
CA
Country
United States
Zip Code
90095
Lee, Frank Y; Wang, Huei-Bin; Hitchcock, Olivia N et al. (2018) Sleep/Wake Disruption in a Mouse Model of BLOC-1 Deficiency. Front Neurosci 12:759
Ghiani, Cristina A; Faundez, Victor (2017) Cellular and molecular mechanisms of neurodevelopmental disorders. J Neurosci Res 95:1093-1096
van Liempd, S M; Cabrera, D; Lee, F Y et al. (2017) BLOC-1 deficiency causes alterations in amino acid profile and in phospholipid and adenosine metabolism in the postnatal mouse hippocampus. Sci Rep 7:5231
Rodriguez-Fernandez, Imilce A; Dell'Angelica, Esteban C (2015) Identification of Atg2 and ArfGAP1 as Candidate Genetic Modifiers of the Eye Pigmentation Phenotype of Adaptor Protein-3 (AP-3) Mutants in Drosophila melanogaster. PLoS One 10:e0143026