The long-term goal of our lab is to understand the molecular mechanisms that control centriole duplication and ensure the accurate distribution of the genome during cell division. Centrioles form the core of centrosomes, which organize the interphase microtubule cytoskeleton of most animal cells and form the poles of the mitotic spindle. Centrioles can also be modified to form basal bodies, which template the formation of cilia and play central roles in cellular signaling, fluid movement, and locomotion. To coordinate these diverse cellular processes, centriole copy number must be precisely controlled. Cells begin G1 phase with two centrioles, which are duplicated during S/G2 phase and are then carefully partitioned into both daughter cells during mitosis. We and others have shown that extra centrioles cause cell division errors that are sufficient to drive malignant transformation. Understanding the mechanism by which cells achieve the once per cycle duplication of the centrioles is, therefore, an important fundamental question of considerable relevance to human health. Although significant progress has been made in understanding the composition of centrioles, it remains unclear which specific steps in centriole formation are controlled by the enzymatic regulator Polo-like-kinase 4 (PLK4). Equally unclear is how a critical regulatory step, termed the ?centriole-to-centrosome conversion? (CCC), functions to maintain centriole homeostasis by enabling new centrioles to acquire competence for duplication. Our proposed research seeks to capitalize on our identification of key interactions and contributors to centriole formation to elucidate molecular mechanisms that control centriole assembly. We will examine three independent components of centriole biogenesis control: the translational regulation of PLK4 expression, the impact of PLK4 phosphorylation of specific substrates in centriole assembly, and the function of a new component required for the CCC.
Aim 1 will define how a pair of conserved upstream open reading frames in the PLK4 mRNA regulate critical aspects of PLK4 expression.
Aim 2 will mechanistically dissect how a critical PLK4 phosphorylation site we identified on STIL controls centriole assembly. Finally, Aim 3 will define how a new centriole protein, PPP1R35, functions to promote the CCC. Understanding how centriole assembly is regulated will reveal fundamental principles of organelle homeostasis and provide insight into the molecular basis of human diseases caused by centriole dysfunction.
Centrioles are organelles with critical roles in cell signaling and cell division. Abnormalities in centriole structure and number are associated with a variety of human diseases including growth defects, microcephaly, and cancer. The work we propose here seeks to define how centrioles are assembled and how their copy number is faithfully controlled, with the goal of elucidating how centriole abnormalities impact human health and disease.
|Levine, Michelle S; Holland, Andrew J (2018) The impact of mitotic errors on cell proliferation and tumorigenesis. Genes Dev 32:620-638|
|Nigg, Erich A; Holland, Andrew J (2018) Once and only once: mechanisms of centriole duplication and their deregulation in disease. Nat Rev Mol Cell Biol 19:297-312|
|Leda, Marcin; Holland, Andrew J; Goryachev, Andrew B (2018) Autoamplification and Competition Drive Symmetry Breaking: Initiation of Centriole Duplication by the PLK4-STIL Network. iScience 8:222-235|
|Lambrus, Bramwell G; Moyer, Tyler C; Holland, Andrew J (2018) Applying the auxin-inducible degradation system for rapid protein depletion in mammalian cells. Methods Cell Biol 144:107-135|
|Evans, Lauren T; Holland, Andrew J (2018) Pushed out of a tough crowd: centrosome aberrations promote invasiveness. EMBO J 37:|
|Lambrus, Bramwell G; Holland, Andrew J (2017) A New Mode of Mitotic Surveillance. Trends Cell Biol 27:314-321|
|Levine, Michelle S; Bakker, Bjorn; Boeckx, Bram et al. (2017) Centrosome Amplification Is Sufficient to Promote Spontaneous Tumorigenesis in Mammals. Dev Cell 40:313-322.e5|
|Levine, Michelle; Holland, Andrew (2017) Cell cycle proteins moonlight in multiciliogenesis. Science 358:716-718|
|Zitouni, Sihem; Francia, Maria E; Leal, Filipe et al. (2016) CDK1 Prevents Unscheduled PLK4-STIL Complex Assembly in Centriole Biogenesis. Curr Biol 26:1127-37|
|Lambrus, Bramwell G; Daggubati, Vikas; Uetake, Yumi et al. (2016) A USP28-53BP1-p53-p21 signaling axis arrests growth after centrosome loss or prolonged mitosis. J Cell Biol 214:143-53|
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