The research goal of this proposal is to understand the genetic variation, evolution, and molecular functions of primary body axis determinants in dipteran insects (midges/mosquitoes and other flies). A new and widely applicable transcriptome-profiling approach was used in preliminary studies to identify structurally unrelated genes and transcript variants that specify head development and embryo polarity in midges (Clogmia albipunctata, Chironomus ripraius), even though their embryos are otherwise genetically and morphologically similar to other flies. The genetic variability of these anterior determinants across species raises questions about evolutionary constraints in fundamental developmental processes of complex organisms and about the developmental consequences when these long-range pattern organizers are substituted. The project combines functional and comparative approaches to examine the mechanisms of action of anterior determinants in dipterans. The first specific aim of this proposal is to improve genetic resources for the prioritized new model organism Clogmia albipunctata, and to examine the evolutionary context in which its newly identified anterior determinant (encoded by Cal-opaC) evolved. This will be done using transcriptome-profiling in related dipteran species, such as sand flies and mosquitoes. The second specific aim is to characterize in vivo the mechanism of action of Cal-opaC and to compare the mechanism of AP axis specification in Clogmia to the respective mechanisms in other lower dipterans, including the midge Chironomus riparius and the soldier fly Hermetia illucens. The outcome will help to understand why key components of some fundamental developmental pathways are more vulnerable to evolutionary change than others and can potentially reveal non-canonical modulation strategies of fundamental signaling pathways that are important for targeted therapeutics in biomedicine.

Public Health Relevance

(Public Relevance Statement) This proposal exploits a new technique with few prerequisites to identify new genes and transcripts with a fundamental and highly specific role in establishing polarity of the primary body axis and takes advantage of newly established experimental model organisms to examine their molecular functions in vivo. Results of this study will help to understand the causes, constraints, and consequences of genetic variation in fundamental genetic mechanisms of complex animals.

Agency
National Institute of Health (NIH)
Institute
National Institute of General Medical Sciences (NIGMS)
Type
Research Project (R01)
Project #
1R01GM127366-01A1
Application #
9612818
Study Section
Development - 2 Study Section (DEV2)
Program Officer
Hoodbhoy, Tanya
Project Start
2018-09-18
Project End
2020-08-31
Budget Start
2018-09-18
Budget End
2019-08-31
Support Year
1
Fiscal Year
2018
Total Cost
Indirect Cost
Name
University of Chicago
Department
Biology
Type
Schools of Medicine
DUNS #
005421136
City
Chicago
State
IL
Country
United States
Zip Code
60637