DYT1 dystonia is a devastating neurological movement disorder characterized by uncontrolled muscle contractions that result in abnormal, involuntary postures. DYT1 dystonia is caused by a deletion (?gag; ?E) in the Tor1A gene encoding the luminal ATPases associated with various cellular activities (AAA+) protein torsinA. How the ?E mutation causes DYT1 dystonia remains unclear because the basic cellular function performed by torsinA is unknown. This proposal seeks to close these critical gaps in knowledge, which will enable the rational design of urgently needed targeted therapies. Our recent work suggests that torsinA is i) required for the transduction of mechanical signals from the cytoskeleton into the nucleoplasm via the nuclear envelope spanning linker of nucleoskeleton and cytoskeleton (LINC) complex; ii) the activation of signaling mediated by the Rho GTPase Cdc42; iii) as well as proper axon outgrowth and growth cone morphology. Importantly, axonal tract disruptions that correlate with clinical severity are observed in the brains of DYT1 dystonia patients and the both the LINC complex and Cdc42 mediate axon elongation and guidance. Thus, we hypothesize that defective torsinA-dependent mechano-chemical signal transduction contributes to DYT1 dystonia pathogenesis. We will test this hypothesis in cultured mammalian cells and the African clawed frog Xenopus laevis using an array of established and novel biochemical, biophysical, fluorescent Rho GTPase biosensors, quantitative imaging and proteomics, as well as synthetic biological approaches. In this proposal, we will define how torsinA and its co-activator, the inner nuclear membrane protein LAP1, regulate the assembly of functional LINC complexes in cultured mammalian cells (Aim 1). We will determine how torsinA and its other co-activator, the outer nuclear membrane protein LULL1, control the activation of Cdc42 in cultured mammalian cells (Aim 2). Finally, we will test the role of torsinA-dependent mechano-chemical signal transduction during axon outgrowth in cultured X. laevis neurons and in the brains of living embryos (Aim 3). The results of these Aims will provide invaluable mechanistic insights into the emerging role of the nuclear envelope as a signaling node in development and disease. Furthermore, they will lay the foundation for the future development of novel therapeutic strategies for the treatment of other forms of dystonia, dystonia plus syndromes in which dystonia can occur in conjunction with another neurological disorder such as Huntington's and Parkinson's diseases, as well as other neurologic and neuropsychiatric diseases caused by mutations in LINC complex proteins including autism, ataxia, bipolar disorder, dementia, and schizophrenia.

Public Health Relevance

/PUBLIC HEALTH RELEVANCE STATEMENT Dystonia is a neurological movement disorder that affects more than 500,000 people in North America, making it the third most prevalent movement disorder behind Parkinson's disease and essential tremor. The most common and severe inherited form is early onset torsion or DYT1 dystonia, which first presents in children at a mean age of ~12 and is caused by a defect in the evolutionarily conserved protein torsinA. This application seeks to elucidate the poorly understood cellular function performed by torsinA, which is necessary for the rational design of improved treatments and potential cures for this debilitating disease.

Agency
National Institute of Health (NIH)
Institute
National Institute of General Medical Sciences (NIGMS)
Type
Research Project (R01)
Project #
5R01GM129374-04
Application #
10248570
Study Section
Nuclear and Cytoplasmic Structure/Function and Dynamics Study Section (NCSD)
Program Officer
Xu, Jianhua
Project Start
2018-09-17
Project End
2022-08-31
Budget Start
2020-09-01
Budget End
2021-08-31
Support Year
4
Fiscal Year
2020
Total Cost
Indirect Cost
Name
University of California Davis
Department
Anatomy/Cell Biology
Type
Schools of Medicine
DUNS #
047120084
City
Davis
State
CA
Country
United States
Zip Code
95618