We will attempt to recapitulate mitochondrial evolution based on the endosymbiotic theory. Eukaryotic organelles, like mitochondria and chloroplasts, are proposed to have evolved from bacterial endosymbionts during an early stage of evolution. Here we will begin to test this theory using two well established model organisms ? E. coli and S. cerevisiae. Specifically, we will generate bacterial endosymbionts in yeast cells. Once we establish stable endosymbionts we will systematically either knockout large parts of the bacterial genome or move specific genes to the host genome to obtain a minimal symbiont genome similar to the early stages of mitochondrial evolution. We will also attempt to further delete genes in the yeast mitochondria, explore those factors that affect the stability of this system and light utilizing endosymbionts using yeast and cyanobacteria. These studies have the potential to provide additional insights into the evolution of complex eukaryotic functions, such as energy generating mitochondria.

Public Health Relevance

We are exploring the evolution of mitochondrial ? the key energy producing organelle in eukaryotes. Our approach involves attempting to recapitulate the endosymbiotic theory of mitochondrial evolution in which protoeukaryotes engulfed prokaryotes and the prokaryote genome subsequently underwent dramatic reduction. This effort will likely provide new insights into evolutionary biology and mitochondrial function.

Agency
National Institute of Health (NIH)
Institute
National Institute of General Medical Sciences (NIGMS)
Type
Research Project (R01)
Project #
1R01GM132071-01
Application #
9706591
Study Section
Synthetic and Biological Chemistry B Study Section (SBCB)
Program Officer
Bond, Michelle Rueffer
Project Start
2019-04-01
Project End
2023-03-31
Budget Start
2019-04-01
Budget End
2020-03-31
Support Year
1
Fiscal Year
2019
Total Cost
Indirect Cost
Name
Scripps Research Institute
Department
Type
DUNS #
781613492
City
La Jolla
State
CA
Country
United States
Zip Code
92037