Abstract

The effect of extracellular matrix (laminin, fibronectin, collagen, proteoglycans) on the differentiation of embryonic corneal epithelium (avian) is being studied. We are especially interested in the effect of these molecules on the configuration of the basal epithelial surface and the organization of the basal epithelial cytoskeleton. We are using heavy meromyosin fragments (S1) to analyse the orientation of actin filaments in cells before and after exposure to matrix molecules. We are using immunohistochemistry and biochemical techniques to follow the differentiation of the epithelium. The effect of matrix molecules on fibroblast shape and migration is also being studied, using similar methods and including Nomarski optics. Avian fibroblasts from cornea, skin, heart, and limb will be compared.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
Eunice Kennedy Shriver National Institute of Child Health & Human Development (NICHD)
Type
Research Project (R01)
Project #
2R01HD000143-26
Application #
3310038
Study Section
Cellular Biology and Physiology Subcommittee 1 (CBY)
Project Start
1975-05-01
Project End
1990-04-30
Budget Start
1985-05-01
Budget End
1986-04-30
Support Year
26
Fiscal Year
1985
Total Cost
Indirect Cost

  Institution

Name
Harvard University
Department
Type
Schools of Medicine
DUNS #
082359691
City
Boston
State
MA
Country
United States
Zip Code

  Publications

Ghetti, Chiara; Gregory, W Thomas; Edwards, S Renee et al. (2005) Severity of pelvic organ prolapse associated with measurements of pelvic floor function. Int Urogynecol J Pelvic Floor Dysfunct 16:432-6
Mattern, R M; Zuk, A; Hay, E D (1993) Retinoic acid inhibits formation of mesenchyme from lens epithelium in collagen gels. Invest Ophthalmol Vis Sci 34:2526-37
Oh, S P; Griffith, C M; Hay, E D et al. (1993) Tissue-specific expression of type XII collagen during mouse embryonic development. Dev Dyn 196:37-46
LuValle, P; Daniels, K; Hay, E D et al. (1992) Type X collagen is transcriptionally activated and specifically localized during sternal cartilage maturation. Matrix 12:404-13
Griffith, C M; Hay, E D (1992) Epithelial-mesenchymal transformation during palatal fusion: carboxyfluorescein traces cells at light and electron microscopic levels. Development 116:1087-99
McCarthy, R A; Hay, E D (1991) Collagen I, laminin, and tenascin: ultrastructure and correlation with avian neural crest formation. Int J Dev Biol 35:437-52
Schoenenberger, C A; Zuk, A; Groner, B et al. (1990) Induction of the endogenous whey acidic protein (Wap) gene and a Wap-myc hybrid gene in primary murine mammary organoids. Dev Biol 139:327-37
Williams, B S; Biggers, J D (1990) Polar trophoblast (Rauber's layer) of the rabbit blastocyst. Anat Rec 227:211-22
Zuk, A; Kleinman, H K; Hay, E D (1989) Culture on basement membrane does not reverse the phenotype of lens derived mesenchyme-like cells. Int J Dev Biol 33:487-90
Hay, E D (1989) Extracellular matrix, cell skeletons, and embryonic development. Am J Med Genet 34:14-29

Showing the most recent 10 out of 22 publications