Abstract

Studies are being performed to elucidate the mechanisms by which the active sliding between flagellar tubules is controlled and coordinated to produce the planar bending waves characteristic of sperm flagella, and to clarify how the dynein arms interact with the axonemal structure and with ATP to produce active sliding between tubules. Analysis of the asymmetrical beating produced by Ca 2 ions has shown that it appears to be the result of the propagation of symmetrical bending waves along a flagellum whose """"""""rest position"""""""" is curved into a nearly circular arc. Electron microscopic studies are being done to determine the subunit structure of the dynein arms. The bending and straightening of flagella in which oscillatory beating is inhibited with vanadate, decreased pH, or Ca 2 ions are being studied to determine the factors that regulate the activity of dynein cross-bridges at different positions along the flagellum, as well as about the properties of the structural components that resist sliding and convert it into bending.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
Eunice Kennedy Shriver National Institute of Child Health & Human Development (NICHD)
Type
Research Project (R01)
Project #
5R01HD006565-19
Application #
3310520
Study Section
Cellular Biology and Physiology Subcommittee 1 (CBY)
Project Start
1977-07-01
Project End
1987-06-30
Budget Start
1985-07-01
Budget End
1986-06-30
Support Year
19
Fiscal Year
1985
Total Cost
Indirect Cost

  Institution

Name
University of Hawaii
Department
Type
Organized Research Units
DUNS #
121911077
City
Honolulu
State
HI
Country
United States
Zip Code
96822

  Publications

Van Berckelaer, Anje C; Mitra, Nandita; Pati, Susmita (2011) Predictors of well child care adherence over time in a cohort of urban Medicaid-eligible infants. BMC Pediatr 11:36
Eshel, D; Shingyoji, C; Yoshimura, K et al. (1992) The phase of sperm flagellar beating is not conserved over a brief imposed interruption. Exp Cell Res 202:552-5
Gibbons, I R; Asai, D J; Tang, W J et al. (1992) A cytoplasmic dynein heavy chain in sea urchin embryos. Biol Cell 76:303-9
Shingyoji, C; Gibbons, I R; Murakami, A et al. (1991) Effect of imposed head vibration on the stability and waveform of flagellar beating in sea urchin spermatozoa. J Exp Biol 156:63-80
Gibbons, I R; Mocz, G (1991) Photocatalytic cleavage of proteins with vanadate and other transition metal complexes. Methods Enzymol 196:428-42
Takahashi, K; Shingyoji, C; Katada, J et al. (1991) Polarity in spontaneous unwinding after prior rotation of the flagellar beat plane in sea-urchin spermatozoa. J Cell Sci 98 ( Pt 2):183-9
Shingyoji, C; Katada, J; Takahashi, K et al. (1991) Rotating the plane of imposed vibration can rotate the plane of flagellar beating in sea-urchin sperm without twisting the axoneme. J Cell Sci 98 ( Pt 2):175-81
Gibbons, I R; Asai, D J; Ching, N S et al. (1991) A PCR procedure to determine the sequence of large polypeptides by rapid walking through a cDNA library. Proc Natl Acad Sci U S A 88:8563-7
Eshel, D; Shingyoji, C; Yoshimura, K et al. (1991) Evidence for an inequality in the forces that generate principal and reverse bends in sperm flagella. J Cell Sci 100 ( Pt 1):213-8
Eshel, D; Shingyoji, C; Yoshimura, K et al. (1990) Transient behavior of sea urchin sperm flagella following an abrupt change in beat frequency. J Exp Biol 152:441-51

Showing the most recent 10 out of 18 publications