The objective of proposed in vivo as well as in vitro nutritional and biochemical research is to continue to define the mechanisms(s) of action of the important micronutrient vitamin A at the molecular level in organs other than the eye. The participation in the mode of vitamin action of two distinct specific intracellular cytoplasmic binding proteins for retionl (vitamin A alcohol) and for retinoic acid (vitamin A acid) purified to homogeneity from rat liver and testis will receive particular attention. In vitro studies are proposed how retinol and retinoic acid bound to their respective binding proteins interact with the cell nucleus changing thereby the genomic expression underlying cell differentiation. The in vivo studies are directed towards the elucidation of factor which regulate the levels of these binding proteins in various organs in adult and in neonatal rats. To this end sensitive radioimmunoassys developed for both binding proteins will be utilized.

Agency
National Institute of Health (NIH)
Institute
Eunice Kennedy Shriver National Institute of Child Health & Human Development (NICHD)
Type
Research Project (R01)
Project #
3R01HD009195-13S1
Application #
3311058
Study Section
Nutrition Study Section (NTN)
Project Start
1988-09-01
Project End
1989-03-31
Budget Start
1988-09-01
Budget End
1989-03-31
Support Year
13
Fiscal Year
1988
Total Cost
Indirect Cost
Name
Vanderbilt University Medical Center
Department
Type
Schools of Medicine
DUNS #
004413456
City
Nashville
State
TN
Country
United States
Zip Code
37203