Abstract

We will investigate biosynthesis by monitoring incorporation of radiolabelled precursors into LH by dispersed rat anterior pituitary cells. Newly synthesized LH will be isolated by immunoprecipitation with specific antibodies. The effects of estrogen and progesterone on LH biosynthesis will be determined in this system. Also the relationships between synthesis and release will be studied in an attempt to determine if these two steps of secretion are tightly coupled. The effect of GnRH on the size of LH will be monitored by comparing LH synthesized in the presence of GnRH with that synthesized in the absence of GnRH.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
Eunice Kennedy Shriver National Institute of Child Health & Human Development (NICHD)
Type
Research Project (R01)
Project #
5R01HD009659-10
Application #
3311142
Study Section
Biochemical Endocrinology Study Section (BCE)
Project Start
1976-05-01
Project End
1987-02-28
Budget Start
1985-09-01
Budget End
1987-02-28
Support Year
10
Fiscal Year
1985
Total Cost
Indirect Cost

  Institution

Name
University of Illinois Urbana-Champaign
Department
Type
Schools of Veterinary Medicine
DUNS #
041544081
City
Champaign
State
IL
Country
United States
Zip Code
61820

  Publications

Liu, T C; Pu, H F; Jackson, G L (1994) Differential actions of phospholipase C on gonadotropin-releasing-hormone-stimulated release and glycosylation of luteinizing hormone in rat anterior pituitary cells. Neuroendocrinology 60:62-8
Jansen, H T; Cooke, P S; Porcelli, J et al. (1993) Estrogenic and antiestrogenic actions of PCBs in the female rat: in vitro and in vivo studies. Reprod Toxicol 7:237-48
Liu, T C; Pu, H F; Jackson, G L (1992) Divergent roles of protein kinase C in luteinizing hormone biosynthesis versus release in rat anterior pituitary cells. Endocrinology 131:2711-6
Liu, T C; Jackson, G L (1990) 17-beta-estradiol potentiates luteinizing hormone glycosylation and release induced by veratridine, diacylglycerol, and phospholipase C in rat anterior pituitary cells. Neuroendocrinology 51:642-8
Liu, T C; Jackson, G L (1989) Differential actions of arachidonic acid and melittin on luteinizing hormone release and synthesis. Neuroendocrinology 49:617-22
Liu, T C; Jackson, G L (1988) Actions of 17 beta-estradiol on gonadotropin release induced by drugs that activate intracellular signal transduction mechanisms in rat anterior pituitary cells. Biol Reprod 39:787-96
Liu, T C; Jackson, G L (1987) Stimulation by phorbol ester and diacylglycerol of luteinizing hormone glycosylation and release by rat anterior pituitary cells. Endocrinology 121:1589-95
Liu, T C; Jackson, G L (1986) Synthesis and release of luteinizing hormone by rat anterior pituitary cells: effects of cytochalasins B and D. Endocrinology 119:236-43
Liu, T C; Jackson, G L (1985) Synthesis and release of luteinizing hormone in vitro: manipulations of Ca2+ environment. Am J Physiol 249:E165-74
Liu, T C; Jackson, G L (1985) Synthesis and release of luteinizing hormone in vitro by rat anterior pituitary cells: effects of gallopamil hydrochloride (D600) and pimozide. Endocrinology 117:1608-14