Reproduction is a complex process regulated by many factors, including the ovarian steroids. Although this process is of obvious importance, it is not adequataly understood in any species. The future success of dealing clinically with the reproductive spectrum, namely infertility and explosive growth rates, depends in part, on a more perfect understanding of the factors controlling the reproductive process. The broad objective of this proposed study is to clarify the interaction of the ovarian steroids with their receptor systems and to determine how this interaction regulates the morphological and biological state of reproductive tract tissue. One of our major aims will be to continue our studies on the effect progesterone has on uteri and oviducts when chronically administered to estradiol-primed cats. This will be accomplished by correlating the plasma levels of estradiol and progesterone, as determined by radioimmunoassay, with alterations in the levels of cytosol and nuclear receptors for estradiol and progesterone, as determined by dextram coated charcoal and exchange assays. The biological response progesterone may induce in uteri and oviducts, in addition to alterations in subcellar compartmentalization of the steroid receptor systems, will be quantitated biochemically by monitoring key enzymes (those involved in glycogen metabolism) amd morphologically by obtaining light and electron micrographs. An additional aim will be to purify a specific uterine protein whose synthesis is induced by estradiol and release is stimulated by progesterone. Our goal is to determine the bioloigical function of this protein and to assess whether it can be as a marker protein to study the mechanism of hormone action at the molecular and genetic level. This study should provide important data in how progesterone controls a) the postovulatory regressive phase of autolysis in the oviduct, and b) the preimplantation progestational phase of the uterus. Additional insight should also be gained into how the uterus supports blastocyst development prior to implantation.

Agency
National Institute of Health (NIH)
Institute
Eunice Kennedy Shriver National Institute of Child Health & Human Development (NICHD)
Type
Research Project (R01)
Project #
5R01HD011404-08
Application #
3311560
Study Section
Reproductive Biology Study Section (REB)
Project Start
1978-01-01
Project End
1987-03-31
Budget Start
1985-04-01
Budget End
1986-03-31
Support Year
8
Fiscal Year
1985
Total Cost
Indirect Cost
Name
University of Illinois at Chicago
Department
Type
Schools of Medicine
DUNS #
121911077
City
Chicago
State
IL
Country
United States
Zip Code
60612
Boomsma, R A; Mavrogianis, P A; Verhage, H G (1997) Immunocytochemical localization of transforming growth factor alpha, epidermal growth factor and epidermal growth factor receptor in the cat endometrium and placenta. Histochem J 29:495-504
Boomsma, R A; Mavrogianis, P A; Fazleabas, A T et al. (1994) Detection of insulin-like growth factor binding protein-1 in cat implantation sites. Biol Reprod 51:392-9
Li, W; Jaffe, R C; Verhage, H G (1992) Immunocytochemical localization and messenger ribonucleic acid levels of a progesterone-dependent endometrial secretory protein (cathepsin L) in the pregnant cat uterus. Biol Reprod 47:21-8
Li, W; Boomsma, R A; Verhage, H G (1992) Immunocytochemical analysis of estrogen and progestin receptors in uteri of steroid-treated and pregnant cats. Biol Reprod 47:1073-81
Li, W G; Jaffe, R C; Fazleabas, A T et al. (1991) Progesterone-dependent cathepsin L proteolytic activity in cat uterine flushings. Biol Reprod 44:625-31
Boomsma, R A; Mavrogianis, P A; Verhage, H G (1991) Changes in endometrial and placental protein synthesis and morphology during pregnancy and pseudopregnancy in the cat. Biol Reprod 44:345-56
Scalzo, C M; Li, W G; Mavrogianis, P A et al. (1990) Immunological detection of cat uterine proteins in ferrets, dogs and baboons. J Reprod Immunol 17:265-77
Jaffe, R C; Donnelly, K M; Mavrogianis, P A et al. (1989) Molecular cloning and characterization of a progesterone-dependent cat endometrial secretory protein complementary deoxyribonucleic acid. Mol Endocrinol 3:1807-14
Verhage, H G; Boomsma, R A; Mavrogianis, P A et al. (1989) Immunological characterization and immunocytochemical localization of a progesterone-dependent cat endometrial secretory protein. Biol Reprod 41:347-54
Verhage, H G; Fazleabas, A T; Mavrogianis, P A et al. (1988) Detection and quantification of CUPED, an estrogen-dependent uterine protein, in uterine fluid and endometrial tissue of estrous and pregnant cats. Am J Anat 181:419-24

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