Abstract

Prostaglandins (PGs) play an important role in the implantation of the blastocyst in the uterus. Blastocysts contain large amounts of PGs in the blastococlic fluid just prior to implantation and release them readily in in vitro culture.
The specific aims of the present proposal are to delineate more specifically the biochemical events in which PGs are involved in the interaction between the blastocyst and the uterus. Both biochemical and morphological studies will be made of the binding, uptake, release and exchange of PGs. Experiments will also determine the extent of synthesis and catabolism of PGs. The tissues to be used will be endometrium, myometrium and blastocysts, mainly as isolated cell preparations, so that each cell type can be studied separately. More information about this process which is crucial to successful reproduction will enable us to develop more effective and specific contraceptive methods.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
Eunice Kennedy Shriver National Institute of Child Health & Human Development (NICHD)
Type
Research Project (R01)
Project #
2R01HD014048-07
Application #
3312444
Study Section
Reproductive Biology Study Section (REB)
Project Start
1980-04-01
Project End
1991-06-30
Budget Start
1987-07-01
Budget End
1988-06-30
Support Year
7
Fiscal Year
1987
Total Cost
Indirect Cost

  Institution

Name
University of Texas Health Science Center San Antonio
Department
Type
Schools of Medicine
DUNS #
800772162
City
San Antonio
State
TX
Country
United States
Zip Code
78229

  Publications

Yang, Z M; Chen, D B; Le, S P et al. (1996) Localization of leukemia inhibitory factor in human endometrium during the menstrual cycle. Early Pregnancy 2:18-22
Yang, Z M; Chen, D B; Le, S P et al. (1996) Differential hormonal regulation of leukemia inhibitory factor (LIF) in rabbit and mouse uterus. Mol Reprod Dev 43:470-6
Yang, Z M; Le, S P; Chen, D B et al. (1995) Leukemia inhibitory factor, LIF receptor, and gp130 in the mouse uterus during early pregnancy. Mol Reprod Dev 42:407-14
Chen, D B; Hilsenrath, R; Yang, Z M et al. (1995) Leukaemia inhibitory factor in human endometrium during the menstrual cycle: cellular origin and action on production of glandular epithelial cell prostaglandin in vitro. Hum Reprod 10:911-8
Kudolo, G B; Yang, Y Q; Chen, D B et al. (1995) Differential metabolism of exogenous platelet-activating factor by glandular epithelial and stromal cells of rabbit endometrium. J Reprod Fertil 105:315-24
Yang, Z M; Chen, D B; Le, S P et al. (1995) Interleukin-1 alpha in the rabbit uterus during early pregnancy. Early Pregnancy 1:201-5
Yang, Z M; Le, S P; Chen, D B et al. (1995) Expression patterns of leukaemia inhibitory factor receptor (LIFR) and the gp130 receptor component in rabbit uterus during early pregnancy. J Reprod Fertil 103:249-55
Kudolo, G B; Harper, M J (1995) Lyso-PAF:acetyl-CoA acetyltransferase and CDP-choline cholinephosphotransferase activities in the rabbit endometrium. J Lipid Mediat Cell Signal 11:145-58
Yang, Z M; Le, S P; Chen, D B et al. (1994) Temporal and spatial expression of leukemia inhibitory factor in rabbit uterus during early pregnancy. Mol Reprod Dev 38:148-52
Norris, C J; Peairs, W A; Kudolo, G B et al. (1994) Platelet-activating factor antagonists and implantation in rabbits. J Reprod Fertil 100:395-401

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