Abstract

I propose to investigate the regulation of form, distribution and polarity of both actin filaments and microtubules in four different cell types. I will continue looking at the acrosomal reactin of Thyone sperm to determine how actin is maintained in an unpolymerized form in unreacted sperm, concentrating on the two high molecular weight proteins. I will carry on the study of the actin filament bundle in Lumulus sperm to try to see how actin elicits motion without myosin and to try to define the structure of F actin. I will look at actin filaments in a naturally occurring actin gel, the cuticular plate of the hair cell of the cochlea, to determine how it is organized and will follow this up with a study of the genesis of this gel. Finally, I propose to examine the assembly and disassembly of microtubules in the axopodia of Echinosphaerium, using fast frozen, deep etched, unfixed, and unextracted cells to determine in vivo exactly how the tubules disassemble and assemble and what the intermediates are and then relate this to what is known from in vitro studies.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
Eunice Kennedy Shriver National Institute of Child Health & Human Development (NICHD)
Type
Research Project (R01)
Project #
2R01HD014474-06
Application #
3312600
Study Section
Cellular Biology and Physiology Subcommittee 1 (CBY)
Project Start
1980-07-01
Project End
1990-06-30
Budget Start
1985-07-01
Budget End
1986-06-30
Support Year
6
Fiscal Year
1985
Total Cost
Indirect Cost

  Institution

Name
University of Pennsylvania
Department
Type
Schools of Arts and Sciences
DUNS #
042250712
City
Philadelphia
State
PA
Country
United States
Zip Code
19104

  Publications

Gedde, M M; Higgins, D E; Tilney, L G et al. (2000) Role of listeriolysin O in cell-to-cell spread of Listeria monocytogenes. Infect Immun 68:999-1003
Hager, K M; Striepen, B; Tilney, L G et al. (1999) The nuclear envelope serves as an intermediary between the ER and Golgi complex in the intracellular parasite Toxoplasma gondii. J Cell Sci 112 ( Pt 16):2631-8
Shaw, M K; Tilney, L G; Musoke, A J et al. (1995) MHC class I molecules are an essential cell surface component involved in Theileria parva sporozoite binding to bovine lymphocytes. J Cell Sci 108 ( Pt 4):1587-96
Shaw, M K; Tilney, L G (1995) The entry of Theileria parva merozoites into bovine erythrocytes occurs by a process similar to sporozoite invasion of lymphocytes. Parasitology 111 ( Pt 4):455-61
Shaw, M K; Tilney, L G; McKeever, D J (1993) Tick salivary gland extract and interleukin-2 stimulation enhance susceptibility of lymphocytes to infection by Theileria parva sporozoites. Infect Immun 61:1486-95
Camilli, A; Tilney, L G; Portnoy, D A (1993) Dual roles of plcA in Listeria monocytogenes pathogenesis. Mol Microbiol 8:143-57
Tilney, L G; Tilney, M S (1993) The wily ways of a parasite: induction of actin assembly by Listeria. Trends Microbiol 1:25-31
Brundage, R A; Smith, G A; Camilli, A et al. (1993) Expression and phosphorylation of the Listeria monocytogenes ActA protein in mammalian cells. Proc Natl Acad Sci U S A 90:11890-4
Tilney, L G; DeRosier, D J; Weber, A et al. (1992) How Listeria exploits host cell actin to form its own cytoskeleton. II. Nucleation, actin filament polarity, filament assembly, and evidence for a pointed end capper. J Cell Biol 118:83-93
Tilney, L G; Tilney, M S; DeRosier, D J (1992) Actin filaments, stereocilia, and hair cells: how cells count and measure. Annu Rev Cell Biol 8:257-74

Showing the most recent 10 out of 33 publications