To study spatial organization and determination in Drosophila embryos, we will characterize embryonic lethal mutations with major zygolic effects on early spatial pattern. A major part of the analysis will concentrate on the two x-linked loci necessary for normal morphogenetic movements at gastrulation. Folded and twisted gastrulation are among the earliest acting genes in Drosophila required within 3 1/2 h after fertilization. We will make amorphic and hypomorphic alleles, determine precise cytogenetic localization, look for intra- and intergenic suppressor mutations and carry out hybrid dysgenesis to obtain alleles useful in the molecular cloning. Morphological studies of sectioned material (light microscopic and EM) will be used to define the cellular defects associated with abnormal morphogenesis. Mosaics produced by ring-x loss and nuclear transportation will be used to define the precise regional requirements for gene activity. We will continue our work on segmentation mutants carrying out detailed analysis of even stripped, odd stripped, armadillo to complement studies of runt and Kruppel. Genetic mosaics will be used to determine cellular autonomy of loci and double mutants will be constructed to detect interactions. We will complete the characterization of the maternal effect mutations in particular those which have effects on early embryonic development. In addition the appropriate maternal loci will be tested for specific interactions with zygolic lethal mutations.

Agency
National Institute of Health (NIH)
Institute
Eunice Kennedy Shriver National Institute of Child Health & Human Development (NICHD)
Type
Research Project (R01)
Project #
5R01HD015587-05
Application #
3313179
Study Section
Genetics Study Section (GEN)
Project Start
1981-08-01
Project End
1989-07-31
Budget Start
1985-08-01
Budget End
1986-07-31
Support Year
5
Fiscal Year
1985
Total Cost
Indirect Cost
Name
Princeton University
Department
Type
Schools of Arts and Sciences
DUNS #
002484665
City
Princeton
State
NJ
Country
United States
Zip Code
Momen-Roknabadi, Amir; Di Talia, Stefano; Wieschaus, Eric (2016) Transcriptional Timers Regulating Mitosis in Early Drosophila Embryos. Cell Rep 16:2793-2801
Falahati, Hanieh; Pelham-Webb, Bobbie; Blythe, Shelby et al. (2016) Nucleation by rRNA Dictates the Precision of Nucleolus Assembly. Curr Biol 26:277-85
Polyakov, Oleg; He, Bing; Swan, Michael et al. (2014) Passive mechanical forces control cell-shape change during Drosophila ventral furrow formation. Biophys J 107:998-1010
He, Bing; Doubrovinski, Konstantin; Polyakov, Oleg et al. (2014) Apical constriction drives tissue-scale hydrodynamic flow to mediate cell elongation. Nature 508:392-6
Khan, Zia; Wang, Yu-Chiun; Wieschaus, Eric F et al. (2014) Quantitative 4D analyses of epithelial folding during Drosophila gastrulation. Development 141:2895-900
Bejsovec, A; Wieschaus, E (1995) Signaling activities of the Drosophila wingless gene are separately mutable and appear to be transduced at the cell surface. Genetics 139:309-20
Postner, M A; Wieschaus, E F (1994) The nullo protein is a component of the actin-myosin network that mediates cellularization in Drosophila melanogaster embryos. J Cell Sci 107 ( Pt 7):1863-73
Bejsovec, A; Wieschaus, E (1993) Segment polarity gene interactions modulate epidermal patterning in Drosophila embryos. Development 119:501-17
Schejter, E D; Wieschaus, E (1993) Functional elements of the cytoskeleton in the early Drosophila embryo. Annu Rev Cell Biol 9:67-99
Schejter, E D; Wieschaus, E (1993) bottleneck acts as a regulator of the microfilament network governing cellularization of the Drosophila embryo. Cell 75:373-85

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