Abstract

The hormone, prolactin, is involved in the development and hormonal differentiation of the mammary gland. In addition, prolactin has been implicated in the growth of mammary tumors. The objective of this study is to delineate the interaction of prolactin with its receptor in order to define the biosites in prolactin and the receptor which are responsible for the mechanism of action of this hormone. The prolactin receptor has been purified to homogeneity and with good yield in this laboratory. Sufficient amounts of receptor from rabbit mammary gland will be isolated and characterized. The amino acid sequence from the N-terminal end will be obtained by gas phase sequencing, and oligonucleotide hybridization probes will be synthesized to be used for identifying clones of the prolactin receptor. The DNA sequence determination will provide the amino acid sequence and the receptor will be expressed in bacteria. The gene for ovine prolactin will be cloned, expressed in bacteria, and the dNA sequence will be determined. Oligonucleotide mutagenesis will be used to alter selected amino acids in both prolactin and the receptor in order to determine their biosites.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
Eunice Kennedy Shriver National Institute of Child Health & Human Development (NICHD)
Type
Research Project (R01)
Project #
5R01HD018584-04
Application #
3315651
Study Section
Biochemical Endocrinology Study Section (BCE)
Project Start
1984-04-01
Project End
1989-03-31
Budget Start
1987-04-01
Budget End
1988-03-31
Support Year
4
Fiscal Year
1987
Total Cost
Indirect Cost

  Institution

Name
University of Kansas
Department
Type
Schools of Medicine
DUNS #
016060860
City
Kansas City
State
KS
Country
United States
Zip Code
66160

  Publications

Hooper, K P; Padmanabhan, R; Ebner, K E (1993) Expression of the extracellular domain of the rat liver prolactin receptor and its interaction with ovine prolactin. J Biol Chem 268:22347-52
Bajpai, A; Hooper, K P; Ebner, K E (1991) Interactions of antisense peptides with ovine prolactin. Biochem Biophys Res Commun 180:1312-7
Bajpai, A; Andrews, G K; Ebner, K E (1989) Induction of c-fos mRNA in rat lymphoma Nb-2 cells. Biochem Biophys Res Commun 165:1359-63
Varma, S; Ebner, K E (1988) The effect of cyclosporin A on the growth and prolactin binding to Nb-2 rat lymphoma cells. Biochem Biophys Res Commun 156:233-9
Andrews, G K; Varma, S; Ebner, K E (1987) Regulation of expression of c-fos and c-myc in rat lymphoma Nb-2 cells. Biochim Biophys Acta 909:231-6
Mahajan, P B; Ebner, K E (1986) Effect of oxidizing agents on rabbit mammary prolactin receptors. Mol Cell Endocrinol 46:37-42